固本平喘方纳米脂质体雾化吸入剂对哮喘豚鼠免疫和基因表达的影响  

Effect of nanoliposome aerosol inhalation of Guben Pingchuan Formula on immunity and gene expression in asthmatic guinea pigs

作　　者：贯剑 宋红普[2] 张彤[2] 张晓晓[3] GUAN Jian;SONG Hongpu;ZHANG Tong;ZHANG Xiaoxiao(Liangyuan Traditional Chinese Medicine Hospital,Shangqu,Henan 476000,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Putuo Hospital Affiliated to Shanghai University of Traditional ChineseMedicine,Shanghai200062,China)

机构地区：[1]河南省商丘市梁园区中医医院,河南商丘476000 [2]上海中医药大学,上海201203 [3]上海中医药大学附属普陀医院,上海200062

出　　处：《上海中医药杂志》2022年第7期96-101,共6页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市科委资助项目(8ZR1416200)。

摘　　要：目的 研究固本平喘方纳米脂质体雾化吸入剂(简称“固本平喘吸入剂”)对哮喘豚鼠免疫和基因表达的影响。方法 将48只豚鼠分为正常对照组、哮喘模型组、中药对照组、固本平喘吸入剂小剂量组(简称“小剂量组”)、固本平喘吸入剂大剂量组(简称“大剂量组”)、西药对照组6组。除正常对照组外,其余各组采用豚鼠腹腔注射含10%卵蛋白的0.9%NaCl溶液方法造模。哮喘模型组、中药对照组、小剂量组、大剂量组、西药对照组分别吸入0.9%NaCl溶液、小青龙合剂、固本平喘吸入剂、地塞米松磷酸钠干预。采用ELISA法检测过敏相关免疫学指标[白介素-4(IL-4)、白介素-5(IL-5)、白介素-12(IL-12)、白介素-25(IL-25)、γ干扰素(IFN-γ)];RT-PCR技术检测信号转导和转录激活因子6(STAT6)、肿瘤坏死因子-α(TNF-α)表达;HE染色观察肺组织病理情况。结果 与正常对照组比较,哮喘模型组IL-4、IL-5、IL-25水平升高,IL-12、IFN-γ水平下降,差异有统计学意义(P<0.05)。与哮喘模型组比较,各药物组IL-4、IL-5水平下降,IL-12、IFN-γ水平升高,同时中药对照组、小剂量组、西药对照组IL-25水平下降,差异有统计学意义(P<0.05)。与正常对照组比较,哮喘模型组STAT6、TNF-α mRNA表达升高,差异有统计学意义(P<0.05)。与哮喘模型组及中药对照组比较,小剂量组及大剂量组STAT6、TNF-α mRNA的表达降低,差异有统计学意义(P<0.05)。与西药对照组比较,小剂量组STAT6、TNF-α mRNA的表达降低,大剂量组TNF-α mRNA的表达降低,差异有统计学意义(P<0.05)。肺组织病理显示:正常对照组由于雾化等因素影响存在肺动脉壁增厚和轻微炎症的情况;哮喘模型组的炎症情况最为严重,动脉壁增厚;中药对照组炎症情况减轻,伴有肉芽组织增生;西药对照组存在肺组织结构受到破坏的情况;小剂量组肺动脉壁增厚,细支气管腔内少量分泌物,3例出现轻度间�Objective Pingchuan Inhaler on immunity and gene expression in asthmatic guinea pigs.Methods normal control group,asthma model group,traditional Chinese medicine(TCM)control group,Guben Pingchuan inhaler low-dose group(hereinafter referred to as low-dose group),Guben Pingchuan Inhaler high-dose group(hereinafter referred to as high-dose group),and western medicine control group.All the groups except for the normal group received an intraperitoneal injection of 10%ovalbumin in 0.9%NaCl solution to make the guinea pig model.Asthma model group,TCM control group,low-dose group,high-dose group,and western medicine control group inhaled 0.9%NaCl solution,Xiaoqinglong Mixture,nano-liposome aerosol inhaler of Guben Pingchuan Formula,and dexamethasone sodium phosphate,respectively.ELISA was used to detect allergy-related immunological indicators[interleukin-4(IL-4),interleukin-5(IL-5),interleukin-12(IL-12),interleukin-25(IL-25),(-)];-transducer and activator of transcription 6(STAT6)and tumor necrosis factor-α(TNF-α)expression;HE staining was used to observe lung histopathology.ResultsThe serum levels of IL-4,IL-5 and IL-25 in the asthma model group were higher and the levels of IL-12 and IFN-γwere lower than those in the normal control group,and the differences were statistically significant(P<0.05).The serum levels of IL-4 and IL-5 were lower and the serum levels of IL-12 and IFN-γ were higher in each drug intervention group than those in the asthma model group.Besides,the levels of IL-25 were lower in the TCM control group,low-dose group and western medicine control group than that in the asthma model group,and the differences were statistically significant(P<0.05).The expression of STAT6 and TNF-αmRNA was higher in the asthma model group than that in the normal control group,and the differences were statistically significant(P<0.05).The expression of STAT6 and TNF-αmRNA was lower in both the low-dose and high-dose groups than that in the asthma model group and the TCM control group,and the differences were stati

关 键 词：哮喘 固本平喘方 雾化吸入 豚鼠 免疫学 基因 中药研究 

分 类 号：R285.5[医药卫生—中药学]