三种方法检验乙型肝炎血清标志物的效果分析  

Analysis of the Effect of Three Methods to Test the Serum Markers of Hepatitis B

作　　者：周毛吉 ZHOU Maoji(Gannan Prefecture People's Hospital,Gannan Prefecture,Gansu Province,747000 China)

机构地区：[1]甘南州人民医院,甘肃甘南州747000

出　　处：《系统医学》2022年第16期51-54,共4页Systems Medicine

摘　　要：目的 评估乙型肝炎血清标志物应用时间分辨萤光分析法(time-resolved fluoroimmunoassay, TRFIA)、电化学发光免疫分析法(electrochemiluminescence immunoassay, ECLIA)、酶联免疫吸附法(enzyme linked immunosorbent assay, ELISA)检验的效果。方法 选择2019年1月—2021年1月期间甘南州人民医院接诊的2 000例乙型肝炎患者,均接受3种诊断方式(TRFIA、ECLIA、ELISA)检测,分析3种检验方式在乙型肝炎血清标志物的诊断效果。结果 TRFIA诊断与ECLIA诊断在HBsAg、HBeAg的阳性率上对比,差异无统计学意义(P>0.05);但是ECLIA诊断在抗HBs、抗HBe、抗HBc的阳性率(59.65%、25.65%、56.00%)明显高于TRFIA诊断(57.50%、25.00%、47.90%),差异有统计学意义(χ^(2)=43.000、11.077、162.000,P<0.001)。TRFIA诊断与ELISA诊断在HBsAg、HBeAg的阳性率上对比,差异无统计学意义(P>0.05);但是TRFIA诊断在抗HBs、抗HBe、抗HBc的阳性率(57.50%、25.00%、47.90%)明显高于ELISA诊断(51.40%、21.55%、43.25%),差异有统计学意义(χ^(2)=122.000、65.000、93.000,P<0.001)。ECLIA诊断与ELISA诊断在HBeAg的阳性率上对比,差异无统计学意义(P>0.05);但是ECLIA诊断在抗HBs、抗HBe、抗HBc的阳性率(59.65%、25.65%、56.00%)明显高于ELISA诊断(51.40%、21.55%、43.25%),差异有统计学意义(χ^(2)=165.000、78.000、255.000,P<0.001)。结论 乙型肝炎血清标志物应用时间分辨萤光分析法、电化学发光法、酶联免疫吸附法检验的效果具有与相似性,临床上可以根据实际情况进行选择。Objective To evaluate the effect of time-resolved fluorescence analysis(TRFIA), electrochemiluminescence immunoassay(ECLIA) and enzyme-linked immunosorbent assay(ELISA) for hepatitis B serum markers. Methods 2000 hepatitis B patients admitted to Gannan Prefecture People’s Hospital from January 2019 to January 2021were selected. All were tested by 3 diagnostic methods(TRFIA, ECLIA, ELISA). The diagnostic effects of the three testing methods on serum markers of hepatitis B were analyzed. Results There was no significant difference in the positive rates of HBsAg and HBeAg between TRFIA diagnosis and ECLIA diagnosis(P>0.05). However, the positive rates of anti-HBs, anti-HBe, and anti-HBc in ECLIA diagnosis(59.65%, 25.65%, 56.00%) were significantly higher than those in TRFIA diagnosis(57.50%, 25.00%, 47.90%), and the difference was statistically significant(χ^(2)=43.000,11.077, 162.000, P<0.001). There was no statistically significant difference in the positive rates of HBsAg and HBeAg between TRFIA diagnosis and ELISA diagnosis(P>0.05). However, the positive rates of anti-HBs, anti-HBe, and anti-HBc in TRFIA diagnosis(57.50%, 25.00%, 47.90%) were significantly higher than those in ELISA diagnosis(51.40%, 21.55%, 43.25%), and the difference was statistically significant(χ^(2)=122.000, 65.000, 93.000, P<0.001).There was no statistically significant difference in the positive rate of HBeAg between ECLIA diagnosis and ELISA diagnosis(P>0.05). However, the positive rates of anti-HBs, anti-HBe, and anti-HBc in ECLIA diagnosis(59.65%,25.65%, 56.00%) were significantly higher than those in ELISA diagnosis(51.40%, 21.55%, 43.25%), and the difference was statistically significant(χ^(2)=165.000, 78.000, 255.000, P<0.001). Conclusion The effects of time-resolved fluorescence analysis, electrochemiluminescence, and enzyme-linked immunosorbent assay for hepatitis B serum markers are similar, and they can be selected according to the actual situation in clinical practice.

关 键 词：时间分辨萤光分析法 电化学发光免疫分析法 酶联免疫吸附法 乙型肝炎血清标志物 

分 类 号：R447[医药卫生—诊断学]