外源NO对钠盐胁迫下苹果砧木M26幼苗光合和生理特性的影响  

Effects of Exogenous NO on Photosynthesis and Physiological Characteristics of Apple Rootstock M26 Seedlings Under Sodium Salt Stress

作　　者：李凯 马利萍 杨天一 张满让[1] LI Kai;MA Liping;YANG Tianyi;ZHANG Manrang(College of Horticulure,Northwest A&F University,Yangling,Shanxi 712100)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《北方园艺》2022年第16期23-30,共8页Northern Horticulture

基　　金：国家现代苹果产业技术体系资助项目(CARS-27)。

摘　　要：以苹果砧木M26幼苗为试材,采用营养液栽培,研究叶片喷施50、100、150、200μmol·L^(-1)和300μmol·L^(-1)5种浓度硝普钠(SNP)作为一氧化氮(NO)供体对盐胁迫下叶片光合色素含量、光合特性、抗氧化物酶、活性氧和膜脂过氧化物质以及渗透调节物质的影响,以期为增强M26幼苗的耐盐性提供参考依据。结果表明:盐胁迫下,M26幼苗叶片的光合色素含量显著降低,光合作用受到抑制,过氧化氢(H_(2)O_(2))和丙二醛(MDA)含量增加,抗氧化酶活性总体上也受到抑制,渗透调节物质含量上升。外源喷施200μmol·L^(-1)的SNP后,M26幼苗的光合色素含量、光合作用效率和抗氧化酶活性均明显提升,H_(2)O_(2)和MDA含量显著降低,可溶性蛋白质和脯氨酸(Pro)含量也得到了进一步提升。可见,外源添加NO可在一定程度上缓解盐胁迫对M26幼苗造成的伤害,从而提高幼苗的耐盐性,促进幼苗生长。Apple rootstock M26 seedlings were grown in nutrient solution to study the effects of five concentrations of SNP(50,100,150,200μmol·L^(-1) and 300μmol·L^(-1)) on chlorophyll content,photosynthetic properties,antioxidant enzymes,reactive oxygen species and membrane lipid peroxides,and osmoregulatory substances under salt stress,in order to provide reference for enhancing the salt tolerance of M26 seedlings.The results showed that the photosynthetic pigment content was significantly reduced,photosynthesis was inhibited,H_(2)O_(2) and MDA content increased,and antioxidant enzyme activity was also inhibited in general,while osmoregulatory substance content increased in M26 seedling leaves under salt stress.After exogenous spraying of 200μmol·L^(-1) SNP,the photosynthetic pigment content,photosynthetic efficiency and antioxidant enzyme activity of M26 seedlings were significantly increased,the contents of H_(2)O_(2) and MDA were significantly decreased,and the contents of soluble protein and Pro were further increased.It could be seen that exogenous addition of NO could alleviate the damage caused by salt stress to M26 seedlings to a certain extent,thus improving the salt tolerance of seedlings and promoting seedling growth.

关 键 词：NO 盐胁迫 苹果砧木 光合特性 生理特性 

分 类 号：S661.1[农业科学—果树学]