实脾饮治疗肝硬化腹水的作用机制研究  

Study on the mechanism of the Shipi decoction on cirrhotic ascites based on network pharmacology

作　　者：张译文 卢秉久[2] ZHANG Yiwen

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中医临床研究》2022年第26期43-46,共4页Clinical Journal Of Chinese Medicine

摘　　要：目的:运用网络药理学方法,探讨实脾饮治疗肝硬化腹水机制。方法:运用TCMSP数据库检索实脾饮中成分与靶点,通过GeneCards和DISGENET数据库查询肝硬化腹水相关靶点。借助Uniprot数据库对检索的靶点进行基因名称校正,将交集靶点输入String数据库,构建蛋白质-蛋白质相互作用网络,通过Metascape数据库进行药物-疾病交集靶点的热图富集总分析,通过David 6.8进行基因本体论(GO)富集分析、京都基因与基因组百科全书(KEGG)通路分析及药物-靶点分析,使用R语言对KEGG富集分析结果进行可视化操作,利用Cytoscape 3.9.0软件,构建成分-靶点-疾病网络,进行拓扑分析。结果:实脾饮中与治疗肝硬化腹水相关预测靶标蛋白共120种。GO富集分析结果显示,生物过程有631个条目,分子功能有107个条目,细胞组分有52个条目。KEGG通路分析结果显示,120个靶点共得到256条KEGG通路。结论:实脾饮治疗肝硬化腹水的作用机制主要与其对RNA、DNA酶转录的正调控有关,涉及癌症信号通路、脂质和动脉粥样硬化信号通路等,主要靶标蛋白为苏氨酸蛋白激酶(Akt Serine/Threonine Kinase 1,AKT1)、17号染色体抑癌基因(Tumor Protein P53,TP53)、肿瘤坏死因子(Tumor Necrosis Factor,TNF)、白细胞介素-6(Interleukin-6,IL-6)等。Objective:To explore the mechanism of the Shipi decoction(实脾饮)on cirrhotic ascites by using the method of network pharmacology.Methods:TCMSP database was used to search the components and targets of the Shipi decoction,and GeneCards and DISGENET database were used to query the related targets of cirrhotic ascites.The gene names of the retrieved targets were corrected with the help of UniProt database,the intersection targets were input into string database,and the protein-protein interaction network was constructed.The heat map enrichment and total analysis of drug disease intersection targets were carried out through metascape database 8 carry out go enrichment analysis,KEGG pathway analysis and drug target analysis.Use R language to visualize the KEGG enrichment analysis results,and use Cytoscape 39.0 software,construct component target disease network,and conduct topology analysis.A total of 120 target proteins in ascites were predicted to be related to the treatment of liver cirrhosis.The results of go enrichment analysis showed that there were 631 entries in biological process,107 entries in molecular function and 52 entries in cell component.KEGG pathway analysis showed that a total of 256 KEGG pathways were obtained from 120 targets.Conclusion:the mechanism of Shipi decoction in the treatment of cirrhotic ascites is mainly related to its positive regulation of RNA and DNase transcription,involving cancer signal pathway,lipid and atherosclerosis signal pathway.The main target proteins are Akt serine/threonine kinase 1,chromosome 17 gene,tumor necrosis factor Interleukin-6,etc..

关 键 词：实脾饮 肝硬化腹水 网络药理学 靶标蛋白预测 

分 类 号：R442.5[医药卫生—诊断学]