水稻NAC转录因子基因OsDSR42的克隆与功能初步分析  

Cloning and Functional Primary Analysis of OsDSR42,a NAC Transcription Factor Gene from Rice

作　　者：黄前量 徐庆国[1] 殷绪明[2] Huang Qianliang;Xu Qingguo;Yin Xuming(College of Agronomy,Hunan Agricultural University,Changsha,410128;Key Laboratory for Agro-ecological Process in Subtropical Region,Institute of Subtropical Agriculture,Changsha,410125)

机构地区：[1]湖南农业大学农学院,长沙410128 [2]中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,长沙410125

出　　处：《分子植物育种》2022年第16期5229-5235,共7页Molecular Plant Breeding

基　　金：国家自然科学基金面上项目(31671612)资助。

摘　　要：干旱胁迫是影响水稻产量和品质的重要因素之一。为获得水稻耐旱功能基因,本研究对NAC转录因子基因OsDSR42进行功能分析。结果发现,在干旱胁迫条件下,水稻中过表达OsDSR42基因能够提高水稻苗期的耐旱性;转基因水稻体内脯氨酸和可溶性糖含量明显高于野生型,而丙二醛和双氧水含量明显低于野生型;此外,转基因水稻中超氧化物歧化酶和过氧化氢酶的活性明显高于对照。以上结果说明,OsDSR42基因可能通过清除干旱胁迫条件下水稻体内积累的活性氧来提高水稻的抗旱性,对以后研究水稻调控应答干旱胁迫的分子机理和培育耐旱水稻新品种具有一定的理论意义和实践意义。Drought has become one of the important factors affecting rice production.To identify the drought-tolerant gene in rice,the function of the plant-specific NAC transcription factor gene OsDSR42 was analyzed.The results of this study showed that overexpressing OsDSR42 could improve drought stress tolerance of transgenic rice at the seedling stage.Compared with control,transgenic rice with overexpression of OsDSR42 resulted in an increase in proline and soluble sugar contents and a decreased malonic dialdehyde and hydrogen peroxide contents exposed to drought stress.And,the SOD and CAT activities were greatly elevated in transgenic plants than those in the wild type.The above results indicated that OsDSR42 gene may improve the drought resistance of rice by scavenging the reactive oxygen species accumulated in Rice under drought stress,which has certain theoretical and practical significance for the future study of the molecular mechanism of rice regulation and response to drought stress and the cultivation of new drought resistant rice varieties.

关 键 词：水稻 NAC转录因子 OsDSR42基因 干旱胁迫 

分 类 号：S511[农业科学—作物学]