沿阶草对二氧化硫暴露及干旱胁迫的胁迫反应和生理特征  

Stress Response and Physiological Characteristics of Ophiopogon japonicus to Sulfur Dioxide Exposure and Drought Stress

作　　者：张志梅 宋大鹏 Zhang Zhimei;Song Dapeng(Exhibition Hall of Agricultural Science and Technology of Henan Province,Zhengzhou Henan 450000,China;Henan Agricultural University,Zhengzhou,450046)

机构地区：[1]河南省农业科学技术展览馆,郑州450000 [2]河南农业大学,郑州450046

出　　处：《分子植物育种》2022年第16期5471-5476,共6页Molecular Plant Breeding

基　　金：河南省软科学研究(222400410567)资助。

摘　　要：本研究试图探讨沿阶草对二氧化硫暴露及干旱胁迫的应激反应和生理特征。将正常培养的沿阶草幼苗分为5组,每组20株。Con组正常培养;Dro组处理期间停止浇水;SO_(2)组幼苗暴露在10 mg/m^(3)的SO_(2)空气中,每天暴露8 h;SO_(2)+Dro组处理期间同时进行干旱和SO_(2)暴露处理;SO_(2)+Dro+HT组在SO_(2)+Dro组处理基础上,每天喷洒3次0.5 mmol/L HT。测定各组沿阶草的生长发育和生理生化指标。研究结果表明:(1)干旱胁迫下,SO_(2)暴露处理能够显著增加沿阶草幼苗成活率、相对含水量和内源性H2S含量(P<0.05);(2)Dro组沿阶草幼苗中脯氨酸含量为(43.67±3.14)μg/g FW,Dro+SO_(2)组中为(48.83±4.26)μg/g FW,显著增加(P<0.05),而可溶性糖含量却显著下降(P<0.05);(3)Dro组沿阶草中SOD、POD和CAT含量分别为(149.41±12.38)U/mg、(141.73±13.25)U/mg、(43.83±3.06)U/mg,Dro+SO_(2)组中分别为(158.52±12.41)U/mg、(175.93±15.61)U/mg、(37.56±2.87)U/mg,差异显著(P<0.05),对MDA含量影响不显著(P>0.05)。总之,本研究发现SO_(2)暴露处理可以增加沿阶草幼苗成活率、相对含水量和内源性H2S含量,提高脯氨酸积累和抗氧化酶类活性,对沿阶草干旱胁迫和二氧化硫暴露的耐受性的进一步研究提供了研究基础。This study attempts to explore the stress response and physiological characteristics of Ophiopogon japonicus to sulfur dioxide exposure and drought stress.The normally cultivated seedlings of O.japonicus were divided into 5 groups with 20 plants in each group.Con group was cultured normally;stop watering during the treatment of Dro group;seedlings in SO_(2) group were exposed to 10 mg/m^(3)SO_(2)air for 8 hours every day;during the treatment of SO_(2)+Dro group,drought and SO_(2) exposure treatment shall be carried out at the same time;in SO_(2)+Dro+ht group,0.5 mmol/L HT was sprayed three times a day based on the treatment of SO_(2)+Dro group.The growth,development and physiological and biochemical indexes of O.japonicus in each group were measured.The results showed that:(1)Under drought stress,SO_(2) exposure treatment could significantly increase the survival rate,relative water content and endogenous H2S content of O.japonicus seedlings(P<0.05);(2)The proline content of O.japonicus seedlings in Dro group was(43.67±3.14)μg/g FW,(48.83±4.26)μg/g FW in Dro+SO_(2)group,which increased significantly(P<0.05),but the soluble sugar content decreased significantly(P<0.05);(3)The contents of SOD,POD and CAT in O.japonicus in Dro group were(149.41±12.38)U/mg,(141.73±13.25)U/mg,(43.83±3.06)U/mg respectively,and(158.52±12.41)U/mg,(175.93±15.61)U/mg,(37.56±2.87)U/mg respectively in Dro+SO_(2) group,with significant difference(P<0.05)and no significant effect on MDA content(P>0.05).In conclusion,this study found that SO_(2) exposure treatment can increase the survival rate,relative water content and endogenous H2S content of O.japonicus seedlings,improve proline accumulation and antioxidant enzyme activities,and provide a research basis for further research on the tolerance of O.japonicus to drought stress and sulfur dioxide exposure.

关 键 词：沿阶草 二氧化硫 胁迫反应 干旱胁迫 

分 类 号：Q945.78[生物学—植物学]