转录组分析揭示热激诱导家蚕孤雌生殖发生相关的信号通路  

Transcriptomic Analysis Reveal the Related Signaling Pathway for Heat Shock-Induced Parthenogenesis in Silkworm Bombyx mori

作　　者：陈金娥[1] 杜鑫[1] 徐霞 于少芳 姚陆松[1] 何秀玲[1] 王永强[1] Chen Jin'e;Du Xin;Xu Xia;Yu Shaofang;Yao Lusong;He Xiuling;Wang Yongqiang(Sericultural and Tea Research Insttute,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江省农业科学院蚕桑与茶叶研究所,杭州310021

出　　处：《蚕业科学》2022年第4期302-311,共10页ACTA SERICOLOGICA SINICA

基　　金：浙江省自然科学基金项目(LY19C170004);国家自然科学基金项目(31902212,32100377);浙江省十四五育种专项(2021C02072);财政部和农业农村部国家现代农业产业技术体系项目。

摘　　要：为了筛选热激诱导家蚕非减数分裂孤雌生殖(ameiotic parthenogenesis)发生相关的基因和关键信号通路,应用Illumina HiSeq X Ten平台对具有高发生率和高孵化率的孤雌生殖系与低发生率和低孵化率的两性生殖系亲本在热激诱导前后的未受精卵进行转录组测序和比较分析。热激诱导前后分别鉴定到差异表达基因4 477与6 196个。热激诱导前差异表达基因主要涉及卵子发生、绒毛膜、卵壳形成等过程,暗示热激前孤雌生殖系和两性生殖系在发育水平上存在差异。热激诱导后差异表达基因主要富集在膜组成部分,参与信号转导通路的差异表达基因最多,主要富集在cAMP、PI3K-Akt、MAPK、Ras等信号通路,这些通路与热激响应和减数分裂有关,推测热激可能通过cAMP等信号通路调控减数分裂过程参与孤雌生殖的发生。本研究有助于进一步探索复杂的孤雌生殖诱导发生机制。In order to screen the genes and key signal pathways related to the heat shock-induced ameiotic parthenogenesis(AMP) in the silkworm, the Illumina HiSeq X Ten platform was used for transcriptomic sequencing and comparative analysis on unfertilized eggs between the parthenogenetic line(PL) with high incidence and high hatchability and the amphigenetic line(AL) with low incidence and low hatchability before and after heat shock induction. Totally 4 477 and 6 196 differentially expressed genes were identified before and after heat shock induction respectively. The differentially expressed genes before heat shock induction were mainly involved in the processes of oogenesis, chorion, and egg shell formation, etc., suggesting differences in the developmental level between PL and AL before heat shock. Differentially expressed genes after heat shock induction were mainly enriched in the membrane components, and most of them were involved in signal transduction pathways which mainly enriched in signal pathways such as cAMP, PI3 K-Akt, MAPK, Ras etc. These pathways were related to the heat shock response and meiosis. It was speculated that heat shock might regulate the meiosis process through signal pathways like cAMP and then participate in the occurrence of parthenogenesis. This study will help to further explore the complicated mechanism of parthenogenesis induction.

关 键 词：家蚕 热激诱导 孤雌生殖 转录组 信号传导通路 CAMP 

分 类 号：S881.2[农业科学—特种经济动物饲养]