川芎提取物及复合配方对睾酮诱导脱发的C57BL/6小鼠的治疗效果和机制研究  

Therapeutic Effect and Mechanism of Ligusticum wallichii Extract and the Prescription on Testosterone⁃induced Hair Loss in C57BL/6 Mice

作　　者：曾泉富 王著显 薛雅琪 张煜盛 朱钊铭 胡义 王媛 吴煜凡 张榕文 刘莉[1] 申春燕 江翠平 沈群[1] 朱红霞[1] ZENG Quanfu;WANG Zhuxian;XUE Yaqi;ZHANG Yusheng;ZHU Zhaoming;HU Yi;WANG Yuan;WU Yufan;ZHANG Rongwen;LIU Li;SHEN Chunyan;JIANG Cuiping;SHEN Qun;ZHU Hongxia(College of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,Guangdong,China;Bawang(Guangzhou)Co.Ltd.,Guangdong Province,Guangzhou 510440)

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]霸王(广州)有限公司,广东广州510440

出　　处：《今日药学》2022年第7期510-515,共6页Pharmacy Today

摘　　要：目的观察中药川芎提取物和复合配方(含川芎,生姜,侧柏叶,人参,丹参,何首乌)局部外用对C57BL/6雄激素性脱发模型小鼠的治疗效果以及机制初探。方法采用背部施加睾酮溶液连续21 d的方法诱导C57BL/6小鼠雄激素性脱发模型,肉眼观察涂抹不同浓度川芎提取物及复合配方物后小鼠背部皮肤的颜色变化及毛发的恢复生长情况;测定给药后小鼠新生毛发的长度、覆盖率评价对毛发生长的影响;通过皮肤测试仪测定小鼠皮肤水分、油分和温度变化情况;通过皮肤病理切片观察各组小鼠皮肤厚度,毛囊形态、大小等组织学变化;ELISA法检测给药后小鼠皮肤中α⁃MSN、TGF⁃β1、VEGF的水平。结果川芎醇提取物能够促进脱发小鼠的毛发生长,低剂量组效果会优于高剂量组及复合配方组;水分、油分和温度值结果显示模型鼠皮肤的水分减少,油分升高,温度略微升高,而经治疗后,小鼠水分、油分和温度恢复到正常值;组织学结果显示川芎和其复合配方能够增加小鼠皮肤厚度、生长期毛囊的数量;川芎高低剂量和复合配方均能不同程度增加小鼠皮肤α⁃MSN、TGF⁃β1、VEGF的含量。结论中药川芎局部应用可以通过恢复脱发模型小鼠的水分,油分和温度、增加皮肤VEGF、α⁃MSN、TGF⁃β1来促进雄激素性脱发小鼠的毛发生长。OBJECTIVE To observe the effects of local application of Ligusticum wallichii extract and its compound extract(including Ligusticum wallichii,Ginger,Platycladus orientalis,Ginseng,Salvia miltiorrhiza,Polygonum multiflorum)on C57BL/6 seborrheic alopecia model mice and then explore the mechanisms involved in it.METHODS The seborrheic alopecia model of C57BL/6 mice were induced by applying testosterone solution on the back for 21 days.After the treatment of different concentrations Ligusticum wallichii extract and its compound extract,the length and coverage of new hair in mice were evaluated for hair growth.Skin moisture,oil and temperature changes were measured by skin test.The histological changes such as skin thickness,hair follicle shape and size of the mice in each group were measured by microscope.The levels ofα⁃MSN,TGF⁃β1 and VEGF in the skin of mice after administration were detected by ELISA.RESULTS The alcohol extract of Ligusticum wallichii promoted the hair growth of alopecia mice,and the effect of the low⁃dose group was better than that of the high⁃dose group and the compound formula group.The results of moisture,oil and temperature values showed that the skin moisture of the model mice was reduced,the oil content was increased,and the temperature was slightly increased.After treatment,the water content,oil content and temperature of mice returned to normal values.Histological results showed that Ligusticum wallichii and its compound formula could increase the skin thickness and the number of hair follicles in the growth phase of mice.Moreover,high and low doses of Ligusticum wallichii and the compound formula could promote the contents ofα⁃MSN,TGF⁃β1 and VEGF in mice skin to different extent.CONCLUSION The topical application of traditional Chinese medicine Ligusticum wallichii can promote hair growth in mice with androgenetic alopecia by restoring moisture,oil and temperature in alopecia model mice,and increasing skin VEGF,α⁃MSN,and TGF⁃β1.

关 键 词：川芎 C57BL/6小鼠 雄激素性脱发 睾酮 

分 类 号：R932[医药卫生—生药学]