2019年广西7份本地感染登革病毒E基因特征分析  

E gene characterization of seven local infections of dengue virus in Guangxi in 2019

作　　者：王静[1] 闭福银[1] 陈华凤[1] 居昱[1] 康宁[1] WANG Jing;BI Fu-yin;CHEN Hua-feng;JU Yu;KANG Ning(Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention,Nanning,Guangxi 530028,China)

机构地区：[1]广西重大传染病防控与生物安全应急响应重点实验室,广西壮族自治区疾病预防控制中心急性传染病防制所,广西南宁530028

出　　处：《现代预防医学》2022年第18期3421-3425,3435,共6页Modern Preventive Medicine

摘　　要：目的了解2019年广西本地感染登革病毒(Dengue virus,DENV)E基因特性,探索可能的输入来源。方法采用实时荧光定量PCR(real-time fluorescence quantitative PCR,RT-qPCR)方法对广西本地感染登革热病例急性期血清样本进行病毒核酸检测并分型,扩增登革病毒E基因后测序,测序结果与不同地区和国家的参考株进行同源性比较和系统进化分析。结果53份登革热病例血清标本经RT-qPCR分型,结果42份(79.2%,42/53)登革病毒核酸阳性,其中南宁9份、梧州10份、玉林17份、崇左6份,均为DENV-1型,其余11份为登革病毒核酸阴性,42份DENV-1型阳性核酸样本经过E基因扩增共得到7份,其中南宁市1份、梧州市1份、玉林市2份、崇左市3份,进化分析结果显示7份样本均为DENV-1型GenotypeⅠ基因型,其中DENV1/GXNN/007/2019、DENV1/GXWZ/009/2019、DENV1/GXYL/011/2019、DENV1/GXYL/012/2019与2019年广东株(序列号:MN921500)同源性99.94%~100.00%,DENV1/GXCZ/015/2019、DENV1/GXCZ/016/2019、DENV1/GXCZ/017/2019与2015年印度尼西亚株(序列号:MG894852)同源性达99.60%,与1945年夏威夷参考株(序列号:AF425619)比较存在12处氨基酸位点变异。结论2019年广西登革病毒是GenotypeⅠ基因型,推测病毒从广东和东南亚国家输入导致的本地流行,应加强登革热跨省、跨境传播的防控。Objective To understand the genetic characteristics of locally infected dengue virus(DENV)E in Guangxi in 2019 and explore possible sources of import.Methods Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect and type viral nucleic acid in serum samples of locally infected dengue cases in Guangxi during the acute phase,and the E gene of dengue virus was amplified and sequenced.The sequencing results were compared with reference strains from different regions and countries for homology and phylogenetic analysis.Results Fifty-three dengue fever case serum specimens were typed by RT-qPCR.There were 42 tested positive for dengue virus(79.2%,42/53),including nine in Nanning,10 in Wuzhou,17 in Yulin,and six in Chongzuo,all of which were DENV-1,and the remaining 11 were tested negative.Among the 42 DENV-1 positive nucleic acid samples,a total of seven copies were obtained after E gene amplification.The results of evolutionary analysis showed that all seven samples were DENV-1 GenotypeⅠgenotypes,including DENV1/GXNN/007/2019,DENV1/GXWZ/009/2019,DENV1/GXYL/011/2019,and DENV1/GXYL/012/2019 which were 99.94 to 100.00%homologous to the 2019 Guangdong strain(sequence number:MN921500),DENV1/GXCZ/015/2019,DENV1/GXCZ/016/2019,and DENV1/GXCZ/017/2019 which were 99.60%homologous to the 2015 Indonesia strain(sequence number:MG894852)and 12 amino acid site variants compared with the 1945 Hawaii reference strain(sequence number:AF425619).Conclusion The 2019 Guangxi dengue virus is GenotypeⅠgenotype speculated to be imported from Guangdong and Southeast Asian countries.The prevention and control of cross-provincial and cross-border transmission of dengue fever should be strengthened.

关 键 词：登革病毒 E 基因 序列分析 系统进化分析、 

分 类 号：R373.33[医药卫生—病原生物学]