响应面法优化超声辅助提取山药素Ⅰ工艺  

Optimization of Ultrasonic-assisted Extraction Process of BatatasinⅠby Response Surface Methodology

作　　者：王晓辉[1] 胡明航 董黎明[1] 李靖[1] 陈艳[1] 李昭[1] 黄菊 WANG Xiaohui;HU Minghang;DONG Liming;LI Jing;CHEN Yan;LI Zhao;HUANG Ju(School of Materials and Chemical Engineering,Xuzhou Institute of Technology,Xuzhou 221018,China)

机构地区：[1]徐州工程学院材料与化学工程学院,江苏徐州221018

出　　处：《徐州工程学院学报（自然科学版）》2022年第3期59-64,共6页Journal of Xuzhou Institute of Technology(Natural Sciences Edition)

基　　金：国家自然科学基金项目(51873232)。

摘　　要：采用响应面法优化、超声辅助提取铁棍山药中山药素Ⅰ,结果表明最佳的提取工艺为:液料比(mL/g)46∶1、乙醇体积分数29%和超声时间63 min.Box-Behnken(BB)中心组合设计试验证明各单因素的影响顺序:乙醇体积分数>超声时间>液料比,液料比和超声时间、乙醇体积分数和超声时间的二次交互作用显著.在最佳条件下进行验证,山药素Ⅰ提取量为0.6271 mg/g,接近模型预测值0.6434 mg/g,响应面法对山药素Ⅰ提取工艺参数优化可行.Response surface methodology and ultrasonic-assisted extraction were used to optimize extraction of batatasinⅠfrom dioscorea opposita.The results showed that the best extraction process was as follows:liquid-solid ratio(mL/g)46∶1,ethanol concentration 29%and ultrasonic time 63 min.Box Behnken(BB)central composite design test showed that the order of influence of each single factor was:ethanol concentration>ultrasonic time>liquid-solid ratio.The secondary interactions between liquid-solid ratio and ultrasonic time,ethanol concentration and ultrasonic time were significant.It was verified under the best conditions that the extraction amount of batatasinⅠwas 0.6271 mg/g,close to the model prediction value of 0.6434 mg/g,and the response surface method was feasible to optimize the extraction process parameters of batatasinⅠ.

关 键 词：铁棍山药 山药素Ⅰ 响应面优化设计 超声辅助 提取 

分 类 号：R284.2[医药卫生—中药学]