趋化因子CXCL1在增殖期婴幼儿血管瘤组织中表达研究及外源性CXCL1对血管瘤干细胞的影响  

Study on the expression of chemokine CXCL1 in infantile hemangioma tissues and the effect of exogenous CXCL1 on hemangioma stem cells

作　　者：翟晓玥 吴瑶 娄寅[1] 谢娟[1] 李红红[1] 曹东升[1] Zhai Xiaoyue;Wu Yao;Lou Yin;Xie Juan;Li Honghong;Cao Dongsheng(Dept of Plastic Surgery,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学第二附属医院整形外科,合肥230601

出　　处：《安徽医科大学学报》2022年第9期1385-1388,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅高校自然科学研究重点项目(编号:KJ2020A184)。

摘　　要：目的探索趋化因子CXCL1在增殖期婴幼儿血管瘤(IH)中的表达情况及外源性CXCL1对血管瘤干细胞(HemSCs)的影响。方法免疫组化法检测CXCL1在增殖期IH标本中的表达情况。通过CD133免疫磁珠将HemSCs原代细胞从增殖期IH组织中分选出。设置5组不同浓度的CXCL1(0、10、20、50、100 ng/ml)与HemSCs进行共培养,通过细胞活率、迁移实验研究外源性CXCL1对HemSCs的影响。结果CXCL1在增殖期IH间质组织中表达,总体表达量低,但在IH瘤内间质组织表达量高于瘤旁间质组织。其中CXCL1阳性面积率瘤内(0.773±0.101)%相比瘤旁(0.268±0.081)%,差异有统计学意义(t=7.843,P<0.001)。外源性CXCL1促进HemSCs增殖,不同浓度CXCL1加入HemSCs中培养24、48、72 h后,差异均有统计学意义(F=14.610,P<0.001;F=14.430,P<0.001;F=5.388,P<0.01)。但加入外源性CXCL1并不影响HemSCs的迁移能力。结论CXCL1在增殖期IH瘤内间质表达高于瘤旁间质,外源性CXCL1可促进HemSCs增殖。Objective To explore the expression of chemokine CXCL1 in proliferative infantile hemangioma(IH),and to study the effect of exogenous CXCL1 on hemangioma stem cells(HemSCs).Methods Immunohistochemistry was used to explore the expression of CXCL1 in proliferative IH specimens.Primary HemSCs were isolated from IH tissues by CD133 magnetic beads.5 groups of CXCL1 with different concentrations(0,10,20,50 and 100 ng/ml)were co-cultured with HemSCs,and the effects of exogenous CXCL1 on HemSCs were studied by cell viability and migration experiments.Results CXCL1 was expressed in the interstitial tissues of proliferative IH.The overall expression of CXCL1 in proliferative IH was low,but the expression of CXCL1 in the proliferative IH interstitial tissues was higher than that of the adjacent interstitial tissues.The CXCL1 positive area rate was(0.773±0.101)%in the tumor compared with(0.268±0.081)%in the adjacent tumor,and the difference was statistically significant(t=7.843,P<0.001).Exogenous CXCL1 promoted the proliferation of HemSCs,and there were statistical differences after adding different concentrations of CXCL1 to HemSCs for 24,48,and 72 h(F=14.610,P<0.001;F=14.430,P<0.001;F=5.388,P<0.01).But the exogenous CXCL1 did not affect the migration ability of HemSCs.Conclusion The expression of CXCL1 in proliferative IH interstitial tissues is higher than that in adjacent interstitial tissues,and exogenous CXCL1 promotes the proliferation of HemSCs.

关 键 词：趋化因子 CXCL1 婴幼儿血管瘤 血管瘤干细胞 增殖 

分 类 号：R62[医药卫生—整形外科]