不同地理来源的拟禾本科根结线虫对温度胁迫的耐受性  

Tolerance of different geographical populations of Meloidogyne graminicola to temperature stress

作　　者：彭思源 喻曼 丁中[1] 阳祝红 叶姗 PENG Siyuan;YU Man;DING Zhong;YANG Zhuhong;YE Shan(College of Plant Protection,Hunan Agricultural University,Changsha 410128,China;Agricultural and Rural Bureau of Cili County,Hunan Province,Zhangjiajie 427200,China)

机构地区：[1]湖南农业大学植物保护学院,长沙410128 [2]湖南省慈利县农业农村局,张家界427200

出　　处：《植物保护》2022年第5期108-115,共8页Plant Protection

基　　金：湖南省植保植检站植物防疫防控科研项目(HNZB202003);公益性行业(农业)科研专项(201503114)。

摘　　要：为明确拟禾本科根结线虫不同地理群体对温度胁迫的耐受性,本文比较了河南新乡、湖南平江、湖南益阳、湖南望城、广西融安、广西临桂、广东湛江、海南临高等8个地理群体的2龄幼虫和卵对0、4℃和37℃的耐受性以及2龄幼虫经4℃预处理5 d后在0℃下的存活率。结果表明:不同地理来源的拟禾本科根结线虫对温度的耐受性存在差异。2龄幼虫在0℃和4℃低温下存放不同时间,其死亡率由低到高为:新乡群体<平江、望城、益阳群体<融安、临桂、湛江、临高群体,表现出随纬度增加耐寒性增强的趋势;不同纬度群体卵在0℃或4℃存放不同时间后其孵化率由高到低为:新乡、平江、望城、益阳、融安、临桂群体>湛江、临高群体。卵经37℃高温处理10 d后,低纬度的临高群体的孵化率明显高于其他纬度较高的地理群体,显示临高群体对高温具有较强的适应性。4℃低温预处理可以提高各群体在0℃下的存活率,湖南平江、望城、益阳群体的存活率提高最明显,表明湖南3个群体对低温具有较高的适应性。In order to understand the differences in temperature tolerance among geographical populations of Meloidogyne graminicola,we collected eight M.graminicola populations from Xinxiang,Henan province,Pingjiang,Yiyang,Wangcheng,Hunan province,Rongan,Lingui,Guangxi Zhuang Autonomous region,Zhanjiang,Guangdong province and Lingao,Hainan province,respectively.The temperature tolerance of second-stage juveniles(J_(2)) and eggs of different geographical populations were investigated at 0,4℃and 37℃,and the survival rate of J_(2) was measured at 0℃after pretreated at 4℃for five days.The results showed that M.graminicola displayed geographical difference in temperature tolerance.At 0℃or 4℃,the cold tolerance of different geographical populations of M.graminicola increased with increasing latitude.The mortality of J_(2) of populations from different latitudes obviously varied,from low to high:Xinxiang population<Pingjiang,Wangcheng,Yiyang populations<Rongan,lingui,Zhanjiang,Lingao populations.The hatching rate of eggs of Xinxiang,Pingjiang,Wangcheng,Yiyang,Rongan and Lingui populations was higher than that from the Zhanjiang,Lingao populations.After exposure to 37℃for 10 days,the egg hatching of Lingao population in lower-latitude was higher than that from other higher-latitude populations,indicating a high adaptability of Lingao population to high temperature.The survival rate of J 2 from different geographical populations at 0℃could be enhanced after low temperature acclimation at 4℃for five days,and the increase of the survival rate of populations from Pingjiang,Wangcheng,Yiyang,Hunan province was obviously higher than that of other populations,suggesting a high adaptability of the three populations to cold exposure.

关 键 词：拟禾本科根结线虫 温度胁迫 耐受性 地理群体 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]