番茄黄化曲叶病毒与番茄褪绿病毒复合侵染对番茄黄化曲叶病毒传播的影响  

Influences of co-infection of tomato yellow leaf curl virus and tomato chlorosis virus on the transmission of tomato yellow leaf curl virus

作　　者：罗瑞雪 赵丹 潘求一 曾颖 张战泓[5] 史晓斌[2] 张德咏[2] 史彩华[1] 刘勇 LUO Ruixue;ZHAO Dan;PAN Qiuyi;ZENG Ying;ZHANG Zhanhong;SHI Xiaobin;ZHANG Deyong;SHI Caihua;LIU Yong(College of Agriculture,Yangtze University,Jingzhou 434000,China;Institute of Plant Protection,Hunan Academy of Agricultural Sciences,Changsha 410125,China;Shuangfeng Bureau of Agriculture and Rural Affairs,Loudi 417700,China;Anxiang Plant Protection and Quarantine Station,Changde 415600,China;Vegetable Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China)

机构地区：[1]长江大学农学院,荆州434000 [2]湖南省农业科学院植物保护研究所,长沙410125 [3]双峰县农业农村局,娄底417700 [4]安乡县植保植检站,常德415600 [5]湖南省农业科学院蔬菜研究所,长沙410125

出　　处：《植物保护》2022年第5期116-121,128,共7页Plant Protection

基　　金：国家自然科学基金(32030088,32072383,31972242,31872932);国家大宗蔬菜产业技术体系(CARS-23-D-02)。

摘　　要：番茄黄化曲叶病毒(tomato yellow leaf curl virus,TYLCV)是一种由烟粉虱传播的单链环状DNA病毒,在田间可与多种病毒发生复合侵染,如番茄褪绿病毒(tomato chlorosis virus,ToCV)等。本文对比了TYLCV单独侵染和TYLCV与ToCV复合侵染对烟粉虱获取和传播TYLCV的影响。结果表明,与取食TYLCV单独侵染的番茄相比,取食复合侵染番茄的烟粉虱对TYLCV的传毒率显著提高,且番茄植株和烟粉虱体内TYLCV的病毒积累量也显著提高。试验结果说明复合侵染会提高烟粉虱的传毒率,促进TYLCV的发生与流行。Tomato yellow leaf curl virus(TYLCV)is a single-strand circular DNA virus transmitted by Bemisia tabaci,which can co-infect plants with multiple plant viruses in the field,such as tomato chlorosis virus(ToCV) and so on.RT-PCR and real-time quantitative PCR were used to clarify the influence of co-infection of TYLCV and ToCV on the acquisition and transmission of TYLCV by B.tabaci in this study.The results showed that,the rate of TYLCV transmited by B.tabaci feeding on the co-infected tomato plants was significantly increased,compared with those B.tabaci feeding on the tomato infected by TYLCV alone,and the virus accumulation of TYLCV in tomato plants and B.tabaci was also significantly improved compared with the tomato plants infected with TYLCV alone.Our results revealed that co-infection of TYLCV and ToCV in the field increased the transmission rate by B.tabaci,and promoted the spread and epidemic of TYLCV.

关 键 词：番茄黄化曲叶病毒 复合侵染 番茄褪绿病毒 烟粉虱 实时荧光定量PCR 

分 类 号：S436.412.11[农业科学—农业昆虫与害虫防治]