贵州八角叶枯病病原菌鉴定及生物学特性研究  

Identification and biological characteristics of a new pathogen causing the leaf blight of star anise in Guizhou province

作　　者：张晓勇 邹东霞 张凤 黄乃秀 廖旺姣 严凯 ZHANG Xiaoyong;ZOU Dongxia;ZHANG Feng;HUANG Naixiu;LIAO Wangjiao;YAN Kai(Guangxi Key Laboratory of Special Non-wood Forest Cultivation&Utilization,Nanning 530002,China;School of Biological Sciences and Technology,Liupanshui Normal University,Liupanshui 553004,China)

机构地区：[1]广西特色经济林培育与利用重点实验室,南宁530022 [2]六盘水师范学院生物科学与技术学院,六盘水553004

出　　处：《植物保护》2022年第5期183-189,共7页Plant Protection

基　　金：广西特色经济林培育与利用重点实验室开放课题(JB-20-03-01);贵州省特色重点实验室建设项目(黔教合KY字[2017]012);贵州省自然科学基金(黔科合J字[2014]7447号);六盘水市科技计划项目(52020-2018-03-07)。

摘　　要：为明确引起贵州八角叶枯病的病原种类及其生物学特性,本研究采用单孢分离法获得3个菌株,经形态学特征鉴定结合ITS、tef1和TUB多基因序列分析,3个菌株均被鉴定为庐山拟盘多毛孢Pestalotiopsis lushanensis,这是该菌在八角上引起叶枯病的首次报道。生物学特性研究表明,该菌菌丝生长和孢子萌发最佳温度为25℃,最适pH为6~7,菌丝致死温度为50℃处理10 min,分生孢子致死温度为55℃处理10 min。The identity and biological characteristics of the pathogen causing leaf blight of star anise(Illicium verum) in Guizhou province were explored in this study.Three strains were obtained by single-spore isolation to be associated with the lesions.Based on their morphological characteristics and multi-locus(ITS,tef1 and TUB) phylogenic data,these strains were identified as Pestalotiopsis lushanensis.This is the first report of I.verum leaf blight caused by P.lushanensis.In culture tests,the optimum conditions for mycelial growth and conidia germination were 25℃ and pH 6-7.The lethal temperatures for mycelium and conidium were 50℃ and 55℃ for 10 min,respectively.

关 键 词：八角 庐山拟盘多毛孢 叶枯病 系统发育分析 生物学特性 

分 类 号：S435.73[农业科学—农业昆虫与害虫防治]