植物诱抗剂AHO联合茎尖培养脱除马铃薯S病毒和马铃薯Y病毒  

Elimination of potato virus S and potato virus Y by plant resistance inducer AHO combined with shoot tip culture

作　　者：张丽珍[1] 赵立华[1] 吴阔[1] 陈永对[1] 苏晓霞[1] 张仲凯[1] 董家红 ZHANG Lizhen;ZHAO Lihua;WU Kuo;CHEN Yongdui;SU Xiaoxia;ZHANG Zhongkai;DONG Jiahong(Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Yunnan Provincial Key Lab of Agricultural Biotechnology,Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture and Rural Affairs,Kunming 650205,China;School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,云南省农业生物技术重点实验室,农业部西南作物基因资源与种质创制重点实验室,昆明650205 [2]云南中医药大学中药学院暨云南省南药可持续利用重点实验室,昆明650500

出　　处：《植物保护》2022年第5期239-245,250,共8页Plant Protection

基　　金：云南省现代农业马铃薯产业技术体系建设项目(2019KJTX003);云岭学者(2016-2020)。

摘　　要：研究了植物诱抗剂3-丙酮基-3-羟基羟吲哚(3-acetonyl-3-hydroxyoxindole,AHO)联合茎尖培养从试管苗中脱除马铃薯S病毒(PVS)、马铃薯Y病毒(PVY)的方法和效率。取PVS侵染的‘定薯3号’和‘定薯4号’以及PVY侵染的‘靖薯3号’和‘靖薯4号’的壮芽,茎尖剥离后培养至4~5个叶片,用100 mg/L植物诱抗剂AHO水剂喷施试管苗,每隔2 d喷施一次,共3次,末次喷施2 d后取茎尖剥离培养,获得再生试管苗。用电子显微镜负染色、ELISA、荧光定量RT-PCR检测再生试管苗的带病毒情况。结果显示,AHO对4个品种的马铃薯试管苗生长无影响,用AHO处理后再茎尖剥离培养,脱毒率均高于未处理的对照;检测结果还显示AHO处理的马铃薯再生苗的带毒量也低于未处理的对照,且随处理次数增加带毒量下降。研究结果表明,利用植物诱抗剂AHO联合茎尖剥离培养方法可以提高脱除PVS、PVY的效率,获得无病毒核心苗。Elimination method and efficiency of potato virus S(PVS) and potato virus Y(PVY) from in vitro plantlets by 3-acetonyl-3-hydroxyoxindole(AHO),a plant resistance inducer,combined with shoot-tip culture were investigated.Shoot tips of PVS-infected ‘Dingshu No.3’ and ‘Dingshu No.4’ and PVY-infected ‘Jingshu No.3’ and ‘Jingshu No.4’ were cultured to 4-5 leaves,respectively.100 mg/L AHO was sprayed on the in vitro plantlets every two days for three times,and shoot tips were cultured at two days after third spray to acquire the regenerated plantlets.Viruses in the regenerated plantlets were detected by electron microscope negative staining,ELISA and real time RT-PCR,respectively.The results showed that AHO had no effect on the growth of potato in vitro plantlets,and the virus-free in vitro plantlets ratio of AHO-treated plantlets was higher than that of untreated control.The test results also showed that the virus relative concentration of regenerated plantlets treated with AHO was lower than that of untreated control,and decreased with the increase of treatment times.The results showed that AHO combined with shoot-tip stripping culture could enhance the efficiency of PVS and PVY elimination,and virus-free stock plantlets could be obtained.

关 键 词：3-丙酮基-3-羟基羟吲哚 茎尖培养 马铃薯S病毒 马铃薯Y病毒 病毒脱除 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]