CMIA法在HBV感染患者血清学标志物水平检测中的应用价值  

Application Value of CMIA in the Detection of Serum Markers in Patients with HBV Infection

作　　者：张楠 ZHANG Nan(Department of Laboratory,Luoyang Third People's Hospital(the First Affiliated Hospital of Luoyang Vocational and Technical College),Luoyang 471002,Henan Province,China)

机构地区：[1]洛阳市第三人民医院(洛阳职业技术学院第一附属医院)检验科,河南洛阳471002

出　　处：《罕少疾病杂志》2022年第10期84-85,106,共3页Journal of Rare and Uncommon Diseases

摘　　要：目的探究微粒子化学发光法(CMIA)在乙型肝炎病毒(HBV)感染患者血清学标志物水平检测中的应用价值。方法选取2019年5月至2021年4月我院406例疑似HBV感染患者,抽取空腹外周静脉血,离心分离血清,分别采用CMIA法、酶联免疫吸附法(ELISA)检测血清乙肝表面抗体(HBsAb)、乙肝表面抗原(HBsAg)、乙肝e抗体(HBeAb)、乙肝e抗原(HBeAg)、乙肝核心抗体(HBcAb)浓度。分析CMIA法、ELISA法检测HBV感染患者血清学标志物变异系数(CV)、符合率、HBsAb、HBsAg回收率、HBsAg最低浓度。结果与ELISA法相比,CMIA法检测HBV感染患者血清学标志物CV值较低,差异化显著(P<0.05);CMIA法与ELISA法检测HBV感染患者血清标志物的符合率为99.51%、99.75%、98.28%、99.75%、98.52%;HBsAb平均回收率为98.37%(96.76%~99.61%),HBsAg平均回收率为97.81%(94.92%~99.45%);CMIA法检测HBsAg最低浓度0.05 IU/mL低于ELISA法0.16 IU/mL,P<0.05。结论CMIA法应用于HBV感染患者血清学标志物水平检测中,检测精密度高,回收率高,准确度高,且用于低水平血清HBsAg检测中,效果良好。Objective To explore the application value of particle chemiluminescence(CMIA)in the detection of serum markers in patients with hepatitis B virus(HBV)infection.Methods 406 patients with suspected HBV infection in our hospital from May 2019 to April 2021 were selected.Fasting peripheral venous blood was extracted and serum was isolated from heart.CMIA and enzyme-linked immunosorbent assay were used respectively(ELISA)detection of serum hepatitis B surface antibody(HBsAb),hepatitis B surface antigen(HBsAg),hepatitis B e antibody(HBeAb),hepatitis B e antigen(HBeAg)and hepatitis B core antibody(HBcAb)concentration.Methods CMIA and ELISA methods were used to detect the variation coefficient of serological markers in patients with HBV infection.Results compared with ELISA,the CV value of serum markers in patients with HBV infection detected by CMIA method was lower and the difference was significant(P<0.05);the coincidence rates of serum markers detected by CMIA method and ELISA were 99.51%,99.75%,98.28%,99.75%and 98.52%,and the average recovery rate of HBsAb was98.37%(96.76%~99.61%),the average recovery of HBsAg was 97.81%(94.92%~99.45%);the minimum concentration of HBsAg detected by CMIA method was 0.05 IU/m L,which was lower than 0.16 IU/mL by ELISA,P<0.05.Conclusion CMIA method has high precision,high recovery and high accuracy in the detection of serum markers in patients with H BV infection,and has good effect in the detection of low-level serum HBsAg.

关 键 词：乙型肝炎病毒 微粒子化学发光法 酶联免疫吸附法 血清学标志物 

分 类 号：R446[医药卫生—诊断学]