浙江省猪链球菌2型人源分离株分子分型特征  

Molecular characteristics of Streptococcus suis type 2 from humans in Zhejiang Province

作　　者：朱水荣[1] 陈建才[1] 吴卓颖 张云怡[1] 方磊 张严峻[1] Zhu Shuirong;Chen Jiancai;Wu Zhuoying;Zhang Yunyi;Fang Lei;Zhang Yanjun(Department of Microbiology,Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China)

机构地区：[1]浙江省疾病预防控制中心微生物检验所,杭州310051

出　　处：《中华传染病杂志》2022年第8期483-489,共7页Chinese Journal of Infectious Diseases

基　　金：中国博士后科学基金(2020T130104ZX)。

摘　　要：目的了解浙江省猪链球菌2型(Streptococcus suis type 2,SS2)的分子分型特征。方法对浙江省2005年1月至2021年7月29株散发SS2人源分离株进行基因分型检测,采用脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)、多位点序列分型(multilocus sequence typing,MLST),以及最小核心基因组(minimum core genome,MCG)群方法,分别进行分型检测。结果29株菌株经PFGE得到10种PFGE图谱带型,3个主要分支簇群,其中21株(72.41%)被分成2个主要分支群,8株(27.59%)呈遗传多样性,相似性为49.7%~94.7%。29株菌株经MLST得到3种序列型,其中ST7型25株(86.21%),ST1型3株(10.34%),ST25型1株(3.45%)。29株菌株得到3个MCG基因型,其中E型12株(41.38%),Group1型16株(55.17%),Group4型1株(3.45%)。结论浙江省SS2主要流行两大克隆群高致病株,少数菌株传播过程中可能存在遗传变异,进化分析呈遗传多样性。Objective To determine the molecular characteristics of Streptococcus suis type 2(SS2)in Zhejiang Province.Methods Twenty-nine SS2 sporadic human isolates in Zhejiang Province from Januery 2005 to July 2021 were genotyped by pulsed-field gel electrophoresis(PFGE),multilocus sequence typing(MLST),and minimum core genome(MCG)sequence typing.Results Among 29 strains,10 PFGE patterns and three main clusters were obtained by PFGE.Twenty-one(72.41%)of the strains were divided into two main branch groups and the remaining eight(27.59%)showed genetic diversity with the similarity ranging from 49.7%to 94.7%.Three sequence types were obtained from 29 strains by MLST,including ST7(86.21%(25/29)),ST1(10.34%(3/29))and ST25(3.45%(1/29)).In addition,three genotypes were obtained from 29 strains by MCG,including genotype E(41.38%(12/29)),genotype group 1(55.17%(16/29))and genotype group 4(3.45%(1/29)).Conclusions Two large clonal groups of highly pathogenic strains of SS2 have been prevalent in Zhejiang Province.A few strains display genetic diversity,indicating genetic variation may exist during transmission.

关 键 词：猪链球菌 多位点序列分型 散发人源分离株 分子分型 电泳 凝胶 脉冲场 最小核心基因组 

分 类 号：R378.12[医药卫生—病原生物学]