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作 者:夏琼琼 尚巍 郑兴灿 张文安 王雅雄 孙永利 李鹏峰 XIA Qiong-qiong;SHANG Wei;ZHENG Xing-can;ZHANG Wen-an;WANG Ya-xiong;SUN Yong-li;LI Peng-feng(North China Municipal Engineering Design&Research Institute Co.Ltd.,Tianjin 300074,China)
机构地区:[1]中国市政工程华北设计研究总院有限公司,天津300074
出 处:《中国给水排水》2022年第17期67-73,共7页China Water & Wastewater
基 金:国家水体污染控制与治理科技重大专项(2017ZX07106005)。
摘 要:针对低浓度条件下厌氧氨氧化工艺难以实现对亚硝酸盐氧化菌(NOB)的抑制,采用连续流悬浮载体一段式全程自养脱氮工艺(CANON),以人工模拟废水为进水,考察了间歇曝气对低氨氮浓度条件下自养脱氮的短期影响,同时分析了反应器内微生物的分布特征。结果表明,当进水NH_(4)^(+)-N浓度为40~50 mg/L时,采用曝气20 min、停止曝气20 min的循环曝气模式,曝气末期溶解氧(DO)浓度控制在1.0~1.3 mg/L,则出水TN、NH_(4)^(+)-N浓度分别为7.8~14.4和1.2~5.9 mg/L;间歇曝气在抑制NOB活性的同时,提高了NH_(4)^(+)-N的转化率,从而提高了系统的脱氮性能。对填料生物膜样品进行16S rDNA宏基因组高通量测序,发现反应器内脱氮细菌主要为Proteobacteria、Planctomycetes和Nitrospirae;间歇曝气时,NOB所在的g_Nitrospira菌属被较为彻底地抑制;Candidatus_Kuenenia对低浓度废水的适应性较强。It is difficult to inhibit nitrite oxidizing bacteria(NOB)in anaerobic ammonia oxidation processes for low ammonia nitrogen wastewater treatment.The short-term effects of intermittent aeration on autotrophic denitrification for simulated low ammonia nitrogen wastewater treatment were investigated in a continuous flow one-stage completely autotrophic nitrogen removal over nitrite(CANON)reactor packed with suspended carrier,and the distribution characteristics of microorganisms in the reactor were analyzed.When the influent NH_(4)^(+)-N was 40-50 mg/L,the cyclic aeration mode with on/off of 20 min/20 min was adopted,and the dissolved oxygen(DO)at the end of aeration was controlled at 1.0-1.3 mg/L,the TN and NH_(4)^(+)-N in effluent were 7.8-14.4 mg/L and 1.2-5.9 mg/L,respectively.Intermittent aeration inhibited the activity of NOB and increased the conversion of NH_(4)^(+)-N,thus improving the denitrification performance of the system.High-throughput 16S rDNA metagenomic sequencing showed that Proteobacteria,Planctomycetes and Nitrospirae were the main denitrification bacteria in biofilm samples from the reactor.Intermittent aeration completely inhibited g_Nitrospira genus belonged to NOB.Candidatus_Kuenenia was more adaptable to survive in low ammonia nitrogen wastewater.
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