甲卡西酮及其代谢物在大鼠体内的分布研究  

Rat-modelled Distribution of Methcathinone and Its Metabolites

作　　者：杨秀秀 王学志 贺强[1,2] 于维光 王凌霄 张潮 王涛[1,2] 傅善林 贠克明[1,2] 尉志文 YANG Xiuxiu;WANG Xuezhi;HE Qiang;YU Weiguang;WANG Lingxiao;ZHANG Chao;WANG Tao;FU Shanlin;YUN Keming;WEI Zhiwen(Shanxi Medical University,Jinzhong 030600,Shanxi,China;Key Laboratory of Ministry of Public Security for Toxicology Analysis,Jinzhong 030600,Shanxi,China;Forensic Science Research Centre,University of Technology Sydney,Sydney 2007,NSW,Australia)

机构地区：[1]山西医科大学,山西晋中030600 [2]法庭毒物分析公安部重点实验室,山西晋中030600 [3]澳大利亚悉尼科技大学法庭科学研究中心,澳大利亚悉尼NSW 2007

出　　处：《刑事技术》2022年第5期497-505,共9页Forensic Science and Technology

基　　金：国家重点研发计划(2018YFC0807403)。

摘　　要：本文旨在建立甲卡西酮大鼠腹腔注射染毒模型,观察甲卡西酮及其代谢物在大鼠体内的分布规律。SD大鼠分别经腹腔注射7.6、15.2、22.8 mg/kg的甲卡西酮,观察中毒症状,分别于注射5、15、30 min后处死解剖,立即取心、肝、脾、肺、肾、脑、胃组织、肌肉、睾丸和心血,高效液相色谱-串联质谱方法检测其中甲卡西酮及其代谢物卡西酮、麻黄碱和伪麻黄碱的含量。结果表明,各样本中甲卡西酮、卡西酮的含量分布特点主要为肾>肺、脾、肝、脑>胃组织、心、肌肉、心血、睾丸;在不同时间下,甲卡西酮的含量分布特点主要为15 min的含量﹥5 min的含量﹥30 min的含量,卡西酮的含量分布特点主要为30 min的含量﹥15min的含量﹥5 min的含量。麻黄碱、伪麻黄碱主要分布在肾、肝、肺,其余组织含量基本相同。甲卡西酮及其代谢物在大鼠体内分布不均,肾检材中含量较高,可作为疑似甲卡西酮死亡案例分析的检材。An intraperitoneal infection model was here established about methcathinone with rat as the experimental animal,and the distribution of both the drug itself and its metabolites was observed in the rat tissues.SD rats,the selected experimental animal,were divided into three groups that were injected of methcathinone via the intraperitoneal route under the respective dose of 7.6 mg/kg,15.2 mg/kg and 22.8 mg/kg and successively monitored of their poisoning symptoms.The rats were put to death at 5 min,15 min and 30 min after drug injection,with their heart,liver,spleen,lung,kidney,brain,stomach,muscle,testicle and heart blood being collected immediately.From the extractions of the above rat tissues,HPLC-MS/MS was to detect the distributed methcathinone and its metabolites:cathinone,ephedrine and pseudoephedrine.Few effects were found of dose on distribution patterns of methcathinone,cathinone,ephedrine and pseudoephedrine among the rat tissues.Kidney was shown of highest concentration for the four tested chemical substances.Within same tissue,methcathinone was always demonstrating highest concentration,leaving cathinone,ephedrine and pseudoephedrine being as the sequential followers.Both methcathinone and cathinone were exceeded over the limit of quantification with the tested blood at each dose,contrasting to ephedrine and pseudoephedrine being under the detection limit any time.The distributed methcathinone was sequentially concentrated as 15 min>5 min>30 min,the three time points for rat execution.Nevertheless,cathinone was shown concentrated at the order of 30 min>15 min>5 min.Ephedrine and pseudoephedrine were found of mainly distributing in kidney,liver and lungs.Therefore,both methcathinone and its metabolites were distributed in imbalance among the rat tissues.Kidney was of harboring concentration-highest methcathinone,eligible as the sample for forensic identification of abusing methcathinone or relevant poisoning death case.

关 键 词：法医毒物分析 甲卡西酮 分布 液相色谱-串联质谱法 

分 类 号：DF795.1[医药卫生—法医学]