亚硝酸盐对氨水法测定碳氧血红蛋白的影响  

Effect of Blood-harbored Nitrite on Determination of Carboxyhemoglobin via Ammonia

作　　者：王乐 刘捷[3] 丁斌 张瑞先 关江玲 滕汉飞 柴盼盼 WANG Le;LIU Jie;DING Bin;ZHANG Ruixian;GUAN Jiangling;TENG Hanfei;CHAI Panpan(Nangjing Forest Police College,Nanjing 210023,China;Yunnan University,Kunming 650500,China;Criminal Technology Detachment of Lanzhou Railway Public Security Division,Lanzhou 730030,China;Criminal Investigation Bureau,Gansu Provincial Public Security Department,Lanzhou 730030,China;Gansu Provincial Third People’s Hospital,Lanzhou 730030,China)

机构地区：[1]南京森林警察学院,南京210023 [2]云南大学,昆明650500 [3]兰州铁路公安处刑事技术支队,兰州730030 [4]甘肃省公安厅刑侦局,兰州730030 [5]甘肃省第三人民医院,兰州730030

出　　处：《刑事技术》2022年第5期512-516,共5页Forensic Science and Technology

基　　金：中央高校基本科研业务费专项资金资助项目(LGYB202101);江苏高校“青蓝工程”资助项目。

摘　　要：探究血液中亚硝酸盐的存在对氨水法测定碳氧血红蛋白的结果影响及影响机理,为使用氨水法测定排除干扰,为准确定性定量测定血液中的碳氧血红蛋白提供参考依据。利用紫外-可见分光光度仪在高级读数模式下测定不含亚硝酸盐的不同碳氧血红蛋白含量系列检材血样,再向该系列检材血样中分别加入不同浓度亚硝酸盐后在同等条件下测定碳氧血红蛋白含量,分析含量变化规律,探究亚硝酸盐对氨水法测定碳氧血红蛋白产生影响的原因。结果表明,血液中亚硝酸盐的存在会导致使用氨水法测定碳氧血红蛋白时出现假阳性并能使碳氧血红蛋白含量较低的阳性检材血样的测定值增大。在使用氨水法对需要进行碳氧血红蛋白测定的血样进行定性定量分析时,应重视可能出现的诸如亚硝酸盐或其他具有类似化学性质的毒物的存在造成的影响,防止误判。Hiemal fire heating and pickles eating could sometimes make carbon monoxide and nitrite co-occurrently appear into some specific living conditions,resulting in the relevant poisoning incidents.However,both the carbon monoxide and nitrite are usually such low of their concentration in blood that it is difficult to judge the true cause of poisoning from just one and/or two of them by the superficial symptoms on corpse because nitrite will affect the accuracy of the commonly-used ammonia method to determine the carboxyhemoglobin harbored with blood.Through exploration of influence of contentdifferent nitrite on ammonia-determination of carboxyhemoglobin and the involving mechanism,this paper was purposed to provide support and principle reference for accurate qualitative and quantitative determination of carboxyhemoglobin via ammonia under elimination of interference from nitrite and other analogous substances.With ultraviolet visible(UVVis)spectrophotometer set at the advanced reading mode,the carboxyhemoglobin was respectively ammonia-determined of its contents in series of blood samples without nitrite and with addition of concentration-various nitrite,hence having the reasons explored into why nitrite influences the determination of carboxyhemoglobin via ammonia and the content threshold of influence.The presence of nitrite in blood does lead to false positive results when the blood-harbored carboxyhemoglobin is determined via ammonia with UV-Vis spectrophotometer,or have the content-low carboxyhemoglobin increased of its determined value.Nevertheless,the carboxyhemoglobin,if high concentration in blood,will receive less influence and just decrease its determined value slightly,thus leaving behind an overall relatively tiny effect.In principle,the content of carboxyhemoglobin is determined via ammonia through the calculation based on subtraction of the absorbance of carboxyhemoglobin at the corresponding wavelength from that of oxygenated hemoglobin.Therefore,with ability of the coexisting nitrite to have the ox

关 键 词：毒物分析 氨水法 亚硝酸盐 碳氧血红蛋白 

分 类 号：DF795.1[医药卫生—法医学]