环介导等温扩增(LAMP)技术在果树病毒病检测中的应用  

Application of Loop-mediated Isothermal Amplification (LAMP) in Virus Disease Detection of Fruit Trees

作　　者：罗丽婷 蒋君梅 李向阳[2] 谢鑫[1] Luo Liting;Jiang Junmei;Li Xiangyang;Xie Xin(Key Laboratory of Agricultural Microbiology,College of Agriculture,Guizhou University,Guiyang,Guizhou 550025,China;Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education,Guizhou University,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州大学农学院/农业微生物特色重点实验室,贵州贵阳550025 [2]贵州大学绿色农药与农业生物工程教育部重点实验室,贵州贵阳550025

出　　处：《山地农业生物学报》2022年第5期43-52,共10页Journal of Mountain Agriculture and Biology

基　　金：贵州省科技计划项目(黔科合支撑[2022]一般091);国家自然科学基金项目(32060614);大学生创新创业训练计划项目(贵大国创字[2021]007)。

摘　　要：病害的早期诊断是防治及阻止病害进一步传播的有效途径。环介导等温扩增技术(Loop-mediated Isothermal Amplification,LAMP)因检测速度快、灵敏度高、特异性强、操作简便等优势,已成为病害诊断的主要手段之一。本文综述了LAMP检测技术在果树(苹果、番木瓜、樱桃、葡萄、西番莲)病毒病检测中的应用,总结目前LAMP技术的发展情况并展望其未来前景,以便促进该技术在植物病毒病检测中的应用。Early diagnosis is an effective way to control plant disease and prevent further spread of disease.Loop-mediated isothermal amplification(LAMP)has become one of the key methods for disease diagnosis with the advantages of rapid detection,high sensitivity,strong specificity,and easy operation.This paper reviewed the application of LAMP detection technology in fruits(apple,papaya,cherry,grape,and passion fruit)virus disease detection,and summarized the current development of LAMP technology as well as its development in the future,which will promote the application of LAMP in plant virus disease detection.

关 键 词：LAMP技术 病毒病 快速检测 

分 类 号：S432[农业科学—植物病理学]