胸腺依赖淋巴细胞亚群和自然杀伤细胞活性检测在不同化疗疗效弥漫大B细胞淋巴瘤患者中的表达分析  

Expression analysis of thymus-dependent lymphocyte subsets and natural killer cell activity detection in patients with diffuse large B-cell lymphoma with different chemotherapy responses

作　　者：李雯雯 周章军 LI Wenwen;ZHOU Zhangjun(Department of Hematology,Xiamen Haicang Hospital,Fujian Procince,Xiamen361000,China)

机构地区：[1]厦门市海沧医院血液科,福建厦门361000

出　　处：《中国当代医药》2022年第27期63-66,共4页China Modern Medicine

摘　　要：目的探讨胸腺依赖淋巴细胞(T淋巴细胞)亚群和自然杀伤(NK)细胞活性检测在不同化疗疗效弥漫大B细胞淋巴瘤(DLBCL)患者中的表达水平。方法选取2017年1月至2021年1月在厦门市海沧医院接受治疗并取得一定疗效的80例DLBCL患者作为研究对象,依据患者治疗效果分为完全缓解组(n=44)和部分缓解组(n=36)。选择同期体检健康对象40例作为对照组。比较疾病组与对照组,以及化疗前后疾病组不同疗效研究对象T淋巴细胞亚群、NK细胞活性检测结果的差异。结果化疗前,完全缓解组、部分缓解组CD3+、CD4+、CD4+/CD8+以及NK细胞活性水平低于对照组,CD8+水平高于对照组,差异有统计学意义(P<0.05)。完全缓解组化疗4个周期后CD3+、CD4+、CD4+/CD8+以及NK细胞活性水平高于化疗前及化疗2个周期后,CD8+水平低于化疗前及化疗2个周期后,差异有统计学意义(P<0.05);部分缓解组化疗前、化疗2、4个周期后CD3+、CD4+、CD8+、CD4+/CD8+以及NK细胞活性水平比较,差异无统计学意义(P>0.05)。结论T淋巴细胞亚群和NK细胞活性在DLBCL患者中存在明显表达异常,且不同疗效反应下其表达水平亦不相同,检测结果有助于对DLBCL患者临床疗效的评估,值得深入研究。Objective To investigate the expression levels of thymus-dependent lymphocyte(T lymphocyte)subsets and natural killer(NK)cell activity in diffuse large B-cell lymphoma(DLBCL)patients with different chemotherapy responses.Methods A total of 80 DLBCL patients who received treatment in Xiamen Haicang Hospital from January 2017 to January 2021 and achieved a certain curative effect were selected as the research subjects,and they were divided into complete remission group(n=44)and partial remission group(n=36).Forty healthy subjects who underwent physical examination during the same period were selected as the control group.The differences in the detection results of T lymphocyte subsets and NK cell activity were compared between the disease group and the control group,as well as the disease group before and after chemotherapy.Results Before chemotherapy,the CD3+,CD4+,CD4+/CD8+and NK cell activity levels in the complete remission group and the partial remission group were lower than those in the control group,and the level of CD8+was higher than that in the control group,and the differences were statistically significant(P<0.05).After 4 cycles of chemotherapy,the CD3+,CD4+,CD4+/CD8+and NK cell activity levels in the complete remission group were higher than those before chemotherapy and after 2 cycles of chemotherapy,and the CD8+level was lower than those before chemotherapy and after 2 cycles of chemotherapy,the differences were statistically significant(P<0.05).There were no significant differences in CD3+,CD4+,CD8+,CD4+/CD8+and NK cell activity levels in the partial remission group before chemotherapy,after 2 and 4 cycles of chemotherapy(P>0.05).Conclusion T lymphocyte subsets and NK cell activity were significantly abnormally expressed in DLBCL patients,and their expression levels were different in response to different curative effects.The test results are helpful for evaluating the clinical curative effect of DLBCL patients and are worthy of further study.

关 键 词：胸腺依赖淋巴细胞亚群 自然杀伤细胞活性 弥漫大B细胞淋巴瘤 表达水平 治疗效果 

分 类 号：R392[医药卫生—免疫学]