猪脾转移因子对猪繁殖与呼吸综合征活疫苗(CH-1R株)免疫抗体的影响  

Effect of pig spleen transfer factor on immune antibody of porcine reproductive and respiratory syndrome attenuated vaccine(CH-1R strain)

作　　者：陈上永 Chen Shangyong(Agricultural and Rural Bureau of Shouning County,Fujian Shouning355500)

机构地区：[1]福建省寿宁县农业农村局,福建寿宁355500

出　　处：《福建畜牧兽医》2022年第5期50-52,共3页Fujian Journal of Animal Husbandry and Veterinary medicine

基　　金：福建省科技计划项目(2021S2003);福州市科技计划项目(2020-PT-149)资助。

摘　　要：本研究旨在明确猪脾转移因子(TF)对猪繁殖与呼吸综合征(PRRS)活疫苗(CH-1R株)免疫抗体的影响,采用具有自主知识产权的中国发明专利制备3批猪脾TF,将PRRS活疫苗(CH-1R株)单独或分别与3批TF联合(单独免疫组、联合免疫组1、联合免疫组2和联合免疫组3)免疫PRRS抗体阴性仔猪,并设立非免疫空白组,采用ELISA方法检测PRRS抗体水平。结果显示,免疫后第7 d、14 d、28 d、42 d和56 d,联合免疫组1、联合免疫组2和联合免疫组3的PRRS抗体阳性率均比单独免疫组高20%~40%。结果表明,TF可以提高PRRS活疫苗(CH-1R株)的免疫抗体水平,为PRRS防控提供参考。In order to determine the effect of pig spleen transfer factor(TF) on the immune antibodies of porcine reproductive and respiratory syndrome(PRRS) attenuated vaccine(CH-1R strain), three batches of pig spleen TF were prepared with Chinese invention patents with independent intellectual property rights. PRRS antibody negative piglets were inoculated with PRRS attenuated vaccine(CH-1R strain) as vaccination alone group, PRRS attenuated vaccine(CH-1R strain) combined with three batches of TF(Co-vaccination group 1, Co-vaccination group 2, Co-vaccination group 3) respectively, and blank group(non-immunized) was set up. PRRS antibody level was detected by ELISA. The results showed that the positive rate of PRRS antibody in Co-vaccination group 1, Co-vaccination group 2, Co-vaccination group 3 were 20%~40% higher than that in vaccination alone group. These findings indicated that TF could improve the immune antibody level of PRRS attenuated vaccine(CH-1R strain). The research provide a reference for the prevention and control of PRRS.

关 键 词：转移因子 猪繁殖与呼吸综合征 CH-1R株 免疫抗体 阳性率 

分 类 号：S858.28[农业科学—临床兽医学]