BA-ELISA法对食品中克百威残留的高效测定  

Efficient determination of carbofuran residues in food by BA-ELISA

作　　者：朱文博 赵凌燕 王敏思 宋洋[1] ZHU Wenbo;ZHAO Lingyan;WANG Minsi;SONG Yang(College of Life Sciences,Tianjin Normal University,Tianjin 300387,China)

机构地区：[1]天津师范大学生命科学学院,天津300387

出　　处：《天津师范大学学报（自然科学版）》2022年第4期59-63,共5页Journal of Tianjin Normal University：Natural Science Edition

基　　金：天津市自然科学基金资助项目(18JCQNJC84400);国家自然科学基金青年科学基金资助项目(31301487);天津市科技计划项目(13JCQNJC14800)。

摘　　要：为了简单、快速、准确地检测水果及其制品中的克百威农药残留,基于单克隆抗体,建立了间接竞争生物素/亲和素酶联免疫分析方法(BA-ELISA).以苹果、苹果汁、橙子、橙汁为样品原材料,采用乙腈提取、旋蒸、甲醇复溶等简单前处理,用0.5%的FG-PBS稀释20倍以基本消除基质影响,用BA-ELISA法测定样品中的克百威含量,同时优化检测条件并对该方法进行评价.结果表明,BA-ELISA检测方法的标准曲线检测限为(0.082±0.002)μg/L,灵敏度为(0.265±0.010)μg/L. 4种水果及其制品中的克百威在3种不同加标浓度水平下的添加回收率为90.20%~99.83%,变异系数为1.8%~6.3%,检测时间缩短至1 h.以气相色谱法作为对照,BA-ELISA法对克百威含量的测定较为准确,可作为水果中检测克百威残留的技术手段.To detect carbofuran pesticide residues in fruit and their products simply, quickly and accurately, an indirect competitive biotin/avidin-enzyme-linked immunosorbent assay(BA-ELISA) was established based on the monoclonal antibody. Apple, apple juice, orange and orange juice were used as raw materials. Simple pretreatment including acetonitrile extraction, rotary steaming and methanol re-dissolution was performed. The influence of the matrix was eliminated by diluting 20 times with 0.5% FG-PBS. The carbofuran content was determined by BA-ELISA. At the same time, the detection conditions were optimized and the method was evaluated. The results showed that the detection limit of the BA-ELISA method is(0.082±0.002)μg/L and the sensitivity is(0.265±0.010)μg/L. The recoveries of carbofuran in the four kinds of fruits and their products were 90.20%-99.83%,the coefficient of variation was 1.8%-6.3%,and the detection time was shortened to 1 h. With the GC method as the control,the BA-ELISA method is proved to be accurate in the determination of carbofuran content. So the BA-ELISA method can be used as a technical means to detect carbofuran residues in fruits.

关 键 词：水果 克百威 快速检测 生物素/亲和素酶联免疫(BA-ELISA) 

分 类 号：S482[农业科学—农药学] TS207.3[农业科学—植物保护]