Integrated engineering of enzymes and microorganisms for improving the efficiency of industrial lignocellulose deconstruction  

作　　者：Guodong Liu Yinbo Qu 

机构地区：[1]State Key Laboratory of Microbial Technology,Shandong University,Qingdao 266237,China [2]National Glycoengineering Research Center,Shandong University,Qingdao 266237,China

出　　处：《Engineering Microbiology》2021年第1期11-20,共10页工程微生物学（英文）

基　　金：supported by the National Key R&D Program of China(2018YFB1501700 and 2018YFA0900500);the Young Scholars Pro-gram of Shandong University(YSPSDU,to G.L.).

摘　　要：Bioconversion of lignocellulosic biomass to fuels and chemicals represents a new manufacturing paradigm that can help address society’s energy,resource,and environmental problems.However,the low efficiency and high cost of lignocellulolytic enzymes currently used hinder their use in the industrial deconstruction of lignocellu-lose.To overcome these challenges,research efforts have focused on engineering the properties,synergy,and production of lignocellulolytic enzymes.First,lignocellulolytic enzymes’catalytic efficiency,stability,and toler-ance to inhibitory compounds have been improved through enzyme mining and engineering.Second,synergistic actions between different enzyme components have been strengthened to construct customized enzyme cocktails for the degradation of specific lignocellulosic substrates.Third,biological processes for protein synthesis and cell morphogenesis in microorganisms have been engineered to achieve a high level and low-cost production of lignocellulolytic enzymes.In this review,the relevant progresses and challenges in these fields are summa-rized.Integrated engineering is proposed to be essential to achieve cost-effective enzymatic deconstruction of lignocellulose in the future.

关 键 词：LIGNOCELLULOSE CELLULASE HEMICELLULASE FUNGI Genetic engineering BIOREFINERY 

分 类 号：F42[经济管理—产业经济]