热射病猪血管内皮细胞损伤的病理变化及相关黏附分子表达  

Pathological changes and expression of related adhesion molecules in vascular endothelial cell injury in heatstroke pigs

作　　者：胡玲玲 倪军[2] 付刚[2] 张静[2] Hu Lingling;Ni Jun;Fu Gang;Zhang Jing(Graduate School,Xinxiang Medical University,Xinxiang 453000,Henan Province,China;The 83^(rd) Army Hospital of Chinese PLA,Xinxiang 453000,Henan Province,China)

机构地区：[1]新乡医学院研究生处,河南省新乡市453000 [2]中国人民解放军陆军第八十三集团军医院,河南省新乡市453000

出　　处：《中国组织工程研究》2023年第17期2644-2650,共7页Chinese Journal of Tissue Engineering Research

基　　金：军队后勤科研计划项目(CLJ20J026),项目负责人:倪军。

摘　　要：背景:热射病是一种死亡率极高的疾病,是由高温引起的广泛内皮细胞损伤及多脏器功能衰竭的临床综合征,常见的并发症是凝血功能异常,而血管内皮病变在重度中暑引发的凝血功能紊乱中发挥关键作用。目的:观察热射病猪血管内皮细胞损伤的病理变化特点及相关黏附分子细胞间黏附分子1的表达。方法:选取12只普通级健康雄性巴马小型猪,体质量15-20 kg,采用随机数字表法分为常温对照组及热射病组,每组6只。热射病组建立高温高湿环境下重度中暑猪模型,而常温对照组小猪置于恒温恒湿气候舱中。采集两组实验前、后静脉血,检测血常规、凝血功能指标;取相同部位的腹主动脉、冠状动脉、肺组织标本,苏木精-伊红染色观察两组标本病理损伤变化;采用免疫组织化学染色对比两组细胞间黏附分子1的表达水平;高倍透射电子显微镜观察冠状动脉及肺组织超微结构变化。结果与结论:①常温对照组实验前、后活化部分凝血活酶时间、凝血酶原时间、血浆D-二聚体、纤维蛋白原降解产物水平及红细胞、血小板计数比较,差异无显著性意义(P>0.05);②热射病组实验后红细胞计数、活化部分凝血活酶时间、凝血酶原时间、血浆D-二聚体、纤维蛋白原降解产物水平高于实验前,且高于常温对照组;血小板计数低于实验前,且低于常温对照组,差异有显著性意义(P<0.05);③苏木精-伊红染色:常温对照组腹主动脉、冠状动脉、肺微血管组织病理结构正常;热射病组腹主动脉内皮细胞轻度水肿,未见明显异常;冠状动脉可见血栓形成、内皮细胞脱落、坏死,数量减少;肺微血管弥漫性出血、内皮细胞数量明显减少;④免疫组织化学染色结果显示:常温对照组腹主动脉、冠状动脉、肺组织观察到内皮细胞浆膜上有数量较多、呈棕黄色线状的细胞间黏附分子1阳性表达;热射病组内皮细胞BACKGROUND:Heatstroke is a highly fatal syndrome characterized by extensive endothelial cell injury and multiple organ failure caused by high temperature.The common complication is abnormal coagulation function,and vascular endothelial disease plays a key role in the coagulation disorder caused by seve re heatstroke.OBJECTIVE:To observe the pathological changes of vascular endothelial cell injury in severe heatstroke pigs and the expression of intercellular cell adhesion molecule-1.METHODS:Twelve healthy male Bama miniature pigs(conventional animals) weighing 15-20 kg were randomly divided into normal temperature control group(n=6) and heatstroke group(n=6).Severe heatstroke pig model was made in the heatstroke group under high tempe rature and humidity environment.The pigs in the normal tempe rature control group were placed in a constant temperature humidity chamber.Venous blood samples before and after the expe riment was collected for blood routine and coagulation function examinations in the two groups.The abdominal aorta,co ronary artery and lung tissue samples at the same position were to ke n,and the pathological changes of the samples in the two groups were obse rved by hematoxylin-eosin staining.Immunohistochemical staining was used to observe the level of inte rcellular adhesion molecule-1 in the two groups.The ultrastructural changes of the coronary arte ry and lung were observed by high-power transmission electron microscope.RESULTS AND CONCLUSION:There were no significant changes in activated pa rtial thromboplastin time,prothrombin time,plasma D-dimer,fibrinogen degradation product level and red blood cell and platelet counts in the normal temperature control group before and after the experiment(P> 0.05).The red blood cell count,activated partial thromboplastin time,prothrombin time,plasma D-dimer and fibrinogen degradation products in the heatstroke group increased significantly after the expe riment and were higher than those in the normal temperature control group.Whereas,the platelet count dec

关 键 词：热射病 血管内皮细胞损伤 细胞间黏附分子1 凝血功能 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R363