跟腱全切断联合皮肤烫伤与单纯跟腱全切断诱导小鼠创伤性异位骨化的比较  

Comparative study of traumatic heterotopic ossification in mice induced by Achilles tenotomy combined with skin burn injury and single Achilles tenotomy

作　　者：王正 陈泓书 易新泽宇 李宗焕[1] Wang Zheng;Chen Hongshu;Yi Xinzeyu;Li Zonghuan(Department of Orthopedics Trauma and Microsurgery,Zhongnan Hospital of Wuhan University,Wuhan 430071,Hubei Province,China;Department of Orthopedics,Huangzhou General Hospital,Huanggang 438000,Hubei Province,China)

机构地区：[1]武汉大学中南医院创伤与显微骨科,湖北省武汉市430071 [2]黄州总医院骨科,湖北省黄冈市438000

出　　处：《中国组织工程研究》2023年第17期2664-2668,共5页Chinese Journal of Tissue Engineering Research

基　　金：武汉大学中南医院优势学科项目(XKJS202006),项目负责人:李宗焕。

摘　　要：背景:目前创伤性异位骨化动物模型主要为跟腱全切断模型,但异位骨形成时间较长、体积较小,并且无法准确地再现临床中大多数创伤性异位骨化病例的全身炎症状态。目的:验证跟腱全切断联合皮肤烫伤建立创伤性异位骨化动物模型的有效性,并将其与单纯跟腱全切断对比,评价两种方法的实用性。方法:利用随机数字表法,将40只雄性C57BL/6小鼠随机分为2组,对照组(n=20)进行单纯跟腱全切断手术,实验组(n=20)进行跟腱全切断手术+背部30%皮肤烫伤处理。记录两组小鼠存活率和跟腱部位手术伤口愈合情况,以及实验组小鼠背部烫伤皮肤恢复情况。术后8周行跟腱micro-CT检查及Masson染色,观察手术部位异位骨形成情况。结果与结论:①术后两组小鼠均无死亡,伤口愈合良好,实验组小鼠烫伤皮肤恢复良好;②micro-CT显示,实验组小鼠全部出现创伤性异位骨化,跟腱部位可见明显的类圆形高密度影,异位骨体积为(2.72±0.04)mm3;对照组仅17只小鼠跟腱部位出现创伤性异位骨化,异位骨体积为(0.65±0.08)mm3;两组小鼠异位骨体积比较差异有显著性意义(P<0.05);③Masson染色显示,两组小鼠形成的异位骨均具有骨小梁和骨髓结构,实验组异位骨面积大于对照组(P<0.05);④结果表明,跟腱全切断联合皮肤烫伤能有效建立小鼠创伤性异位骨化模型,与单纯跟健全切断相比,该方法不仅能较早诱导小鼠创伤性异位骨化,且成功率更高,所形成的异位骨体积更大,是动物创伤性异位骨化模型建立的理想方法。BACKGROUND:At present,Achilles tenotomy model is an animal model mainly used for traumatic heterotopic ossification.However,this method requires a long time to form ectopic bone,and the size of the formed ectopic bone is always small.In addition,this method cannot accurately reproduce the systemic inflammatory state of most traumatic heterotopic ossification cases in clinic practice.OBJECTIVE:To verify the validity of the animal model of traumatic heterotopic ossification induced by Achilles tenotomy combined with skin burn injury,to compare this approach with single Achilles tenotomy,and to evaluate the practicability of the two methods.METHODS:Forty male C57BL/6 mice were randomly divided into two groups:Achilles tenotomy group(control group,n=20)and Achilles tenotomy+30%skin scald on the back group(experimental group,n=20).The survival rate and healing of surgical incisions of the mice in the two groups were recorded.Survival rate and wound healing in the two groups as well as skin recovery of burn injury in the experimental group were recorded.Micro-CT examination and Masson staining of the Achilles tendon was performed 8 weeks after surgery to observe the ectopic bone at the surgical site.Formation of ectopic bone was also observed in the two groups.RESULTS AND CONCLUSION:There was no death and wound infection in the two groups.The skin burn injury in the experimental group recovered well without ulceration.Micro-CT findings indicated that all mice in the experimental group developed traumatic heterotopic ossification,with obvious circular high-density shadow at the surgical site,and the volume of ectopic bone was(2.72±0.04)mm3.In contrast,only 17 mice developed traumatic heterotopic ossification in the control group,and the volume of ectopic bone was(0.65±0.08)mm3.There was a significant difference in the volume of ectopic bone between the two groups(P<0.05).Masson staining showed that ectopic bone in both groups had bone trabecular and bone marrow structures,but the area of ectopic bone in the experiment

关 键 词：创伤性异位骨化 动物模型 跟腱切断 皮肤烫伤 炎症 

分 类 号：R459.9[医药卫生—治疗学] R-332[医药卫生—临床医学] R686.5