结合非靶向代谢组学和指纹图谱对通体结香技术产沉香与野生沉香化学成分的比较分析  

Comparative analysis of chemical constituents between the agarwood produced by Whole-tree agarwood-inducing technique and natural agarwood based on the combination of non-targeted metabolomics and fingerprint

作　　者：郁萌 侯文成 冯剑[1] 陈兰 杨云[1] 刘洋洋[1,2] 魏建和 YU Meng;HOU Wen-cheng;FENG Jian;CHEN Lan;YANG Yun;LIU Yang-yang;WEI Jian-he(Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine&Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization,Hainan Branch of the Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Haikou 570311,China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education&National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所海南分所,海南省南药资源保护与开发重点实验室,国家中医药管理局沉香可持续利用重点研究室,海南海口570311 [2]中国医学科学院北京协和医学院药用植物研究所,中草药物质基础与资源利用教育部重点实验室,濒危药材繁育国家工程实验室,北京100193

出　　处：《中草药》2022年第18期5625-5635,共11页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划项目(2018YFC1706403);海南省自然科学基金高层次人才项目(2019RC340);现代农业产业技术体系建设专项(CARS-21);中国医学科学院医学与健康科技创新工程项目(2016-I2M-2-003);2021年国家药品标准提高课题(2021Z14)。

摘　　要：目的 系统全面分析比较通体结香技术产沉香(简称通体香)与野生沉香化学成分,为通体结香技术进一步的应用和推广提供科学可靠的依据。方法 采用超高效液相色谱串联四级杆飞行时间质谱(UPLC-Q/TOF-MS)和气相色谱-质谱(GC-MS)结合非靶向代谢组学和指纹图谱,对通体香与野生沉香的乙醇提取物和挥发性成分进行分析。结果 16批通体香与16批野生沉香的共有成分相同,但通体香指纹图谱相似度较高,且4个倍半萜、3个小分子芳香物质和8个2-(2-苯乙基)色酮等共17个共有成分在通体香中含量较高。结论 通体香和野生沉香主要化学成分组成相同,但不同批次间通体香的质量更稳定。Objective To provide scientific basis for the advanced application and popularization of agarwood produced by Whole-tree agarwood-inducing technique(Agar-Wit), the compositional differences between agarwood induced by Agar-Wit and natural agarwood were analyzed and compared in the study. Methods The ethanol extracts and volatile components of samples were analyzed by ultra-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry(UPLC-Q/TOF-MS) and gas chromatography-mass spectrometry(GC-MS) combined with non-targeted metabolomics and fingerprint in this study. Results The common components of agarwood induced by Agar-Wit were consistent with natural agarwood. The similarity coefficients among Agar-Wit agarwood were higher than that in natural agarwood. In addition, the content of three low-molecular-weight aromatic compounds, four kinds of sesquiterpenes and eight 2-(2-phenylethyl)chromones were richer in agarwood induced by Agar-Wit. Conclusion The main chemical composition of agarwood induced by Agar-Wit, which is more stable, is proved to be in accordance with wild agarwood.

关 键 词：沉香 通体结香技术 2-(2-苯乙基)色酮 非靶向代谢组学 指纹图谱 2 4-二叔丁基苯酚 6 7-二甲基-1 2 3 5 8 8a-六氢萘 去氢蜂斗菜酮 

分 类 号：R284.1[医药卫生—中药学]