基于“心藏神”探讨金丝桃苷对心肌梗死后抑郁大鼠的治疗作用及机制  

Exploration of the Therapeutic Effect and Its Mechanism of Hyperoside on Rats with Post-myocardial Infarction Depression Based on the Concept of“Heart Storing Spirit”

作　　者：裴玲军 王小玲 张乃中 章艺子 李洁 王会敏 裴金娜 唐新花 PEI Ling-Jun;WANG Xiao-Ling;ZHANG Nai-Zhong;ZHANG Yi-Zi;LI Jie;WANG Hui-Min;PEI Jin-Na;TANG Xin-Hua(Qian’an Health Care Center for Old Cadres,Qian’an 064400 Hebei,China;Qian’an Hospital of Traditional Chinese Medicine,Qian’an 064400 Hebei,China)

机构地区：[1]迁安市老干部保健中心,河北迁安064400 [2]迁安市中医医院,河北迁安064400

出　　处：《广州中医药大学学报》2022年第10期2347-2353,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：河北省中医药管理局科研项目(编号:2019243)。

摘　　要：【目的】基于“心藏神”探讨金丝桃苷对心肌梗死后抑郁大鼠的治疗作用及机制。【方法】将60只大鼠随机分为6组,即假手术组,模型组,金丝桃苷低、中、高剂量组和西药(福辛普利钠)组,每组10只。除假手术组外,其余大鼠均建立心肌梗死后抑郁模型。干预结束后,记录大鼠睡眠潜伏期、睡眠时间,酶联免疫吸附分析(ELISA)检测血清白细胞介素(IL)-1β、脑源性神经营养因子(BDNF)及皮质酮(CORT)水平,进行电生理监测,苏木素-伊红(HE)染色法检测脑、心肌组织病理形态,蛋白免疫印迹法检测心肌组织N-甲基-D-天门冬氨酸受体1(NMDAR1)、A型钾离子通道电压门控型钾通道4.2(Kv4.2)蛋白表达水平。【结果】与假手术组比较,模型组大鼠睡眠潜伏期延长、睡眠时间缩短,血清IL-1β和BDNF含量降低、CORT含量升高,梗死周边区心室有效不应期(VERP)升高、心室颤动阈值(VFT)降低,心肌组织中NMDAR1蛋白表达水平升高、Kv4.2蛋白表达水平降低(均P<0.05);与模型组比较,金丝桃苷低、中、高剂量组和西药组大鼠睡眠潜伏期缩短、睡眠时间延长,血清IL-1β和BDNF含量升高、CORT含量降低,梗死周边区VERP降低、VFT升高,心肌组织中NMDAR1蛋白表达水平降低、Kv4.2蛋白表达水平升高(均P<0.05),呈剂量依赖性;与西药组比较,金丝桃苷高剂量组上述指标差异无统计学意义(P>0.05)。【结论】金丝桃苷能够改善心肌梗死后抑郁大鼠心电不稳定性,改善睡眠质量,其机制可能与增加血清中IL-1β、BDNF含量,减少CORT含量,抑制心肌组织NMDAR1表达及促进Kv4.2表达有关。Objective To explore the therapeutic effect and mechanism of hyperoside on rats with post-myocardial infarction depression based on the concept of“heart storing spirit”.Methods Sixty rats were randomly divided into six groups,namely,the sham operation group,the model group,the hyperoside low-,medium-and high-dose groups and the western medicine(fosinopril sodium)group,with 10 rats in each group.The post-myocardial infarction depression model was established in all rats except the sham operation group.After ending the intervention,the sleep latency and sleep time of rats were recorded,serum interleukin(IL)-1β,brain-derived neurotrophic factor(BDNF)and corticosterone(CORT)levels were detected by enzyme-linked immunosorbent assay(ELISA),electrophysiological monitoring was performed,brain and myocardial histopathological morphological features were observed by hematoxylin-eosin(HE)staining,and the protein expression levels of Nmethyl-D-aspartate receptor 1(NMDAR1)and A-type potassium channel voltage-gated potassium channel 4.2(Kv4.2)in cardiac muscular tissues were detected by Western Blot.Results Compared with the sham operation group,rats in the model group had prolonged sleep latency,shortened sleep time,decreased serum IL-1βand BDNF levels,increased CORT levels,increased VERP and decreased VFT in the peri-infarct area,increased protein expression level of NMDAR1 and decreased protein expression level of Kv4.2 in myocardial tissues(all P<0.05);compared with the model group,rats in the hyperoside low-,medium-and high-dose groups and the western medicine group showed shorter sleep latency and longer sleep time,serum IL-1βand BDNF levels were increased,CORT level was decreased,VERP was decreased and VFT was increased in the peri-infarct area,and the protein expression level of NMDAR1 was decreased and Kv4.2 protein expression level was increased in myocardial tissues(all P<0.05),in a dose-dependent manner;compared with the western medicine group,the differences of the above indexes in the high-dose group of hy

关 键 词：金丝桃苷 心藏神 心肌梗死后抑郁 睡眠 电生理 N-甲基-D-天门冬氨酸受体1(NMDAR1) 大鼠 

分 类 号：R285.5[医药卫生—中药学]