细胞骨架相关蛋白PDLIM5在人成纤维细胞内的定位及调控作用  

Localization and regulation of cytoskeleton associated proteins PDLIM5 in human fibroblasts

作　　者：黄小兰 彭彦 杨宇超 樊庭宇 孙冰 Asmat Ullah Khan 戴景兴 欧阳钧 钟世镇 Huang Xiaolan;Peng Yan;Yang Yuchao;Fan Tingyu;Sun Bing;Asmat Ullah Khan;Dai Jingxing;Ouyang Jun;Zhong Shizhen(Department of Human Anatomy,Guangdong Provincial Key Laboratory of Medical Biomechanics,School of Basic Medical Science,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学基础医学院人体解剖学教研室暨广东省医学生物力学重点实验室,广州510515

出　　处：《中国临床解剖学杂志》2022年第5期536-540,共5页Chinese Journal of Clinical Anatomy

基　　金：国家重点研发计划(2017YFC1105000);深圳市医疗卫生三名工程(SZSM201612019)。

摘　　要：目的 研究细胞骨架相关蛋白PDLIM5在人成纤维细胞内的定位及其调控作用。方法通过慢病毒转染下调PDLIM5在人成纤维细胞中的表达后,进行CCK8细胞增殖实验、细胞划痕实验、Transwell迁移实验来检测PDLIM5对细胞增殖和运动迁移能力的影响,通过免疫荧光染色来观察PDLIM5的亚细胞定位。结果 下调PDLIM5表达后抑制了人成纤维细胞的增殖、运动和迁移能力,免疫荧光发现PDLIM5主要表达在细胞质并定位于微丝。结论 PDLIM5定位于细胞骨架微丝,下调PDLIM5表达可抑制人成纤维细胞的增殖、运动和迁移能力。Objective To investigate the localization and regulation of cytoskeleton-related proteins PDLIM5 in human fibroblasts.Methods After the expression of PDLIM5 was down-regulated by lentivirus transfection in human fibroblasts,CCK8 cell proliferation assay,cell scratch assay and Transwell migration assay were performed to detect the effect of PDLIM5 on cell proliferation and migration ability.Immunofluorescence staining was used to observe the cellular localization of PDLIM5.Results Downregulating of PDLIM5 expression inhibited,the proliferation and migration ability of human fibroblasts.Immunofluorescence staining showed that PDLIM5 was mainly expressed in the cytoplasm and localized in the microfilament.Conclusions PDLIM5 is localized in cytoskeleton microfilaments and down-regulation of PDLIM5 expression can inhibit cell proliferation and migration ability of human fibroblasts.

关 键 词：人成纤维细胞 PDLIM5 微丝 细胞骨架相关蛋白 

分 类 号：Q245[生物学—细胞生物学]