基于大科学装置的空间金属组学和单细胞/单颗粒金属组学  

Large Research Infrastructures Based Spatial Metallomics and Single-cell/Single-particle Metallomics

作　　者：范玉芹 崔丽巍 王黎明[3] 赵甲亭 李柏[3] 李玉锋[3] FAN Yuqin;CUI Liwei;WANG Liming;ZHAO Jiating;LI Bai;LI Yu-Feng(Shandong Provincial Maternal and Child Health Care Hospital,Jinan,Shandong 250014,China;College of Sources and Environment,University of Chinese Academy of Sciences,Beijing 100049,China;CAS-HKU Joint Laboratory of Metallomics on Health and Environment,&CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,&Beijing Metallomics Facility,&National Consortium for Excellence in Metallomics,Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]山东省妇幼保健院,济南250014 [2]中国科学院大学资源与环境学院,北京100049 [3]中国科学院高能物理研究所中国科学院-香港大学金属组学与健康和环境联合实验室/中国科学院纳米生物效应与安全性重点实验/北京金属组学平台,北京100049

出　　处：《中国无机分析化学》2022年第4期63-74,共12页Chinese Journal of Inorganic Analytical Chemistry

基　　金：环境地球化学国家重点实验室开放课题(SKLEG2022212)。

摘　　要：金属组学是综合研究生命体内(特别是细胞内)自由或络合的全部金属原子的分布、含量、化学种态及其功能的一门学科,而大科学装置为金属组学研究提供了强有力的工具。首先介绍了金属组学发展简史,然后介绍了基于大科学装置的同步辐射技术、中子技术、质子技术及缪子技术等,最后概述了基于大科学装置的空间金属组学、单细胞/单颗粒金属组学的应用示例。基于大科学装置的中子活化技术(NAA)、X-射线荧光光谱(XRF)及质子激发X射线谱(PIXE)等技术是开展非原位空间金属组学研究的有力手段,而XRF、PIXE及缪子X射线荧光谱(MXA)为开展原位空间金属组学提供了有力工具,特别是基于XRF的技术,其空间分辨率可低至10 nm级别,是开展原位单细胞/单颗粒金属组学的利器。新一代同步辐射光源、质子源及缪子源将为空间金属组学、特别是时空金属组学研究提供更强有力工具。Metallomics is the systematic study of metallome and the interactions and functional connections of metal ions and their species with genes,proteins,metabolites and other biomolecules within organisms and ecosystems.Large research infrastructures(LRIS)can provide cutting-edge techniques for metallomics.In this review,the development of metallomics was first briefly presented.Then,LRI-besed synchrotron radiation,neutron,proton and muonic techniques were introduced.Finally,the application of LRI-based techniques in spatial metallomics,single-cell/single-particle metallomics was summarized.It is noticed that neutron activation analysis(NAA),X-ray fluorescence analysis(XRF)and proton induced X-ray emission(PIXE)can be applied in ex-situ spatial metallomics,while XRF,PIXE and muonic X-ray analysis(MXA)are well-suitable for in-situ spatial metallomics.The XRF with spatial resolution down to 10 nm is capable for single-cell/single-particle metallomics.The new generation of synchrotron radiation sources,proton sources and muonic sources will provide more novel tools for metallomics,especially the spatiotemporal metallomics.

关 键 词：大科学装置 空间金属组学 单细胞金属组学 单颗粒金属组学 时空金属组学 

分 类 号：O655[理学—分析化学] TG1[理学—化学]