基于低分散激光剥蚀系统-电感耦合等离子体飞行时间质谱的快速元素成像  

Fast Elemental Bio-Imaging with Low Dispersion Laser Ablation System Coupled to Inductively Coupled Plasma Time-of-Flight Mass Spectrometry

作　　者：李冬月 郑令娜[2] 常盼盼 汪冰[2] 竺云 王萌[2] 丰伟悦[2] LI Dongyue;ZHENG Lingna;CHANG Panpan;WANG Bing;ZHU Yun;WANG Meng;FENG Weiyue(College of Physics and Materials Science,Tianjin Normal University,Tianjin 300387,China;Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]天津师范大学物理与材料科学学院,天津300387 [2]中国科学院高能物理研究所中科院纳米生物效应与安全性重点实验室,北京100049

出　　处：《中国无机分析化学》2022年第4期142-147,共6页Chinese Journal of Inorganic Analytical Chemistry

基　　金：国家自然科学基金资助项目(11975251,11875268);国家重点研发计划项目(2016YFA0201604)。

摘　　要：微量元素在生物体内含量很低,但发挥着重要的作用。因此,生物体内微量元素原位成像具有重要的意义。建立了基于低分散激光剥蚀系统-电感耦合等离子体飞行时间质谱(LA-ICP-TOFMS)的快速生物元素成像方法,采用点扫描模式,得到了尾静脉注射银纳米颗粒的小鼠肾脏切片中19种元素成像图,成像分辨率为20μm,成像速度为每秒40个像素点,与常规LA-ICP-MS成像速度相比提高了约10倍。成像结果表明,不同元素在小鼠肾脏各分区中具有不同的分布模式。建立的LA-ICP-TOFMS成像方法为原位研究生物体内元素提供了直观可靠的手段,有望在生物医学研究中得到更广泛的应用。Trace elements play important roles in an organism.Therefore,in situ imaging of trace elements is of great significance.A fast element bio-imaging method was developed based on a low-dispersion laser ablation system and inductively coupled plasma time-of-flight mass spectrometry(LA-ICP-TOFMS).Using a spot ablation mode,the images of 19 elements in a kidney section of a mouse intravenously injected with silver nanoparticles was obtained.The spatial resolution of the images was 20μm and the imaging speed was 40 pixels per second,which is about 10 times higher than that of conventional LA-ICP-MS system.The imaging results showed that the distribution patterns of the elements in the mouse kidney were different.The developed LA-ICP-TOFMS method provides a visual and reliable means for in situ study of trace elements in an organism and is expected to be more widely used in biomedical research in the future.

关 键 词：电感耦合等离子体飞行时间质谱 成像质谱流式 低分散剥蚀池 银纳米颗粒 空间金属组 

分 类 号：O657.63[理学—分析化学] TH843[理学—化学]