Microfabricated platforms to investigate cell mechanical properties  

作　　者：Amir M.Esfahani Grayson Minnick Jordan Rosenbohm Haiwei Zhai Xiaowei Jin Bahareh Tajvidi Safa Justin Brooks Ruiguo Yang 

机构地区：[1]Department of Mechanical and Materials Engineering,University of Nebraska-Lincoln,Lincoln,NE,68516,USA [2]Nebraska Center for Integrated Biomolecular Communication,University of Nebraska-Lincoln,Lincoln,NE,68588,USA [3]Mary and Dick Holland Regenerative Medicine Program,University of Nebraska Medical Center,Omaha,NE,68198,USA [4]Department of Bioengineering,School of Medicine,Johns Hopkins University,Baltimore,MD,21205,USA

出　　处：《Medicine in Novel Technology and Devices》2022年第1期49-58,共10页医学中新技术与新装备（英文）

基　　金：We acknowledge the funding support from the NSF(Awards 1826135,1936065);the NIH National Institutes of General Medical Sciences P20GM113126,and P30GM127200;We acknowledge funding support from the Nebraska Collaborative Initiative and EPSCoR FIRST award.G.M.and J.R.are funded by the NSF Graduate Research Fellowship(Awards 2034837,2034837).

摘　　要：Mechanical stimulation has been imposed on living cells using several approaches.Most early investigations were conducted on groups of cells,utilizing techniques such as substrate deformation and flow-induced shear.To investigate the properties of cells individually,many conventional techniques were utilized,such as AFM,optical traps/optical tweezers,magnetic beads,and micropipette aspiration.In specific mechanical interrogations,microelectro-mechanical systems(MEMS)have been designed to probe single cells in different interrogation modes.To exert loads on the cells,these devices often comprise piezo-electric driven actuators that attach directly to the cell or move a structure on which cells are attached.Uniaxial and biaxial pullers,micropillars,and cantilever beams are examples of MEMS devices.In this review,the methodologies to analyze single cell activity under external loads using microfabricated devices will be examined.We will focus on the mechanical interrogation in three different regimes:compression,traction,and tension,and discuss different microfabricated platforms designed for these purposes.

关 键 词：BIOMEMS Single cell Mechanical properties MICRODEVICES 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]