外源水杨酸诱导香蕉苯丙烷类代谢提高对枯萎病抗性  

Exogenous Salicylic Acid Induced Phenylpropane Metabolism in Banana to Improve the Resistance Against Fusarium Wilt

作　　者：段雅婕[1] 杨宝明[2] 郭志祥[2] 尹可锁[2] 胡会刚[1] 曾莉[2] 白亭亭[2] DUAN Yajie;YANG Baoming;GUO Zhixiang;YIN Kesuo;HU Huigang;ZENG Li;BAI Tingting(South Subtropical Crops Research Institute,Chinese Academy of Tropical Agriculture Sciences/Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture and Rural Affairs/Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products,Zhanjiang,Guangdong 524091,China;Agricultural Environment and Resources Institute,Yunnan Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province,Kunming,Yunnan 650205,China)

机构地区：[1]中国热带农业科学院南亚热带作物研究所/农业农村部热带果树生物学重点实验室/海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江524091 [2]云南省农业科学院农业环境资源研究所/云南省农业跨境有害生物绿色防控重点实验室,云南昆明650205

出　　处：《热带作物学报》2022年第9期1870-1879,共10页Chinese Journal of Tropical Crops

基　　金：国家重点研发计划项目(No.2019YFD1000903);财政部和农业农村部国家现代农业产业技术体系项目(No.CARS-31);广东省自然科学基金项目(No.2018A030313602)

摘　　要：由尖孢镰刀菌古巴专化型热带4号生理小种(Fusarium oxysporum f.sp.cubense tropical race 4,TR4)引起的香蕉枯萎病严重阻碍我国香蕉产业的绿色可持续发展。外源水杨酸(SA)处理能诱导香蕉体内SA合成及信号传导途径关键酶基因上调表达,激活香蕉植株内系统获得抗性,增强对各种生物和非生物胁迫的抵抗能力,在此基础上,本研究对香蕉感病品种‘巴西蕉’和抗病品种‘农科1号’进行SA诱导处理,随后接种TR4,结果显示2个品种的病情指数均降低。为了进一步探究水杨酸信号途径下游的苯丙烷类途径在SA诱导的香蕉植株抗病过程中的作用,本研究运用实时荧光定量PCR技术(RT-qPCR),对SA处理的感病品种‘巴西蕉’和抗病品种‘农科1号’中苯丙烷类途径关键基因的表达情况进行分析,结果发现分支途径中常规苯丙烷类途径关键酶基因C4H和4CL、木质素合成途径关键酶基因CAD和CCoAOMT、黄酮类物质生物合成途径关键酶基因CHS和CHI,对SA处理和TR4接种均有响应。在仅接种TR4的情况下,‘巴西蕉’相关基因主要表现为显著下调表达,而‘农科1号’相关基因主要表现为上调表达;仅用SA处理时,2个品种的C4H和4CL被诱导上调表达;SA处理并接种TR4,2个品种C4H和4CL依然保持上调表达的趋势,变化幅度在‘农科1号’中偏高;‘巴西蕉’CAD经SA诱导上调表达,但在接种TR4后,CAD相对表达量降低,而‘农科1号’CAD被SA诱导显著上调表达,SA和TR4共同作用下,表达水平亦显著升高;‘巴西蕉’CCoAOMT被TR4诱导显著上调表达,在SA处理并接种TR4的‘巴西蕉’植株中也表现为上调表达,‘农科1号’CCoAOMT在SA处理或SA、TR4共同作用下表现为强烈上调表达;CHS和CHI在SA处理的2个品种中主要表现为上调表达,SA和TR4双重作用下,依然表现为上调表达。结果表明外源SA处理可诱导感病和耐病香蕉根组织中苯丙烷类途径关键酶基因�Banana Fusarium wilt caused by Fusarium oxysporum f.sp.cubense tropical race 4(TR4)seriously hinders the green and sustainable development of banana industry in China.Exogenous salicylic acid(SA)treatment can induce the key enzyme genes up-regulation in SA synthesis and signaling pathway and further activate systemic resistance in bananas against a variety of biotic and abiotic stresses.In this research,the susceptible banana cultivar‘Brazilian’and resistant banana cultivar‘Nongke No.1’after treated with SA were inoculated with TR4.The results showed that the disease index of the two cultivars treated with SA decreased when it was compared with the SA-untreated control.In order to explore the role of phenylpropanoid pathway located downstream of SA synthesis and signaling pathway during banana resistance induced by exogenous SA against Fusarium wilt,the key genes’expression of phenylpropanoid pathway in susceptible and resistant banana cultivars treated with SA was analyzed by fluorescence quantitative PCR(RT-qPCR).It was found that the key genes C4H and 4CL in general phenylpropanoid pathway were responsive to both SA induction and TR4 inoculation.The same was true for CAD and CCoAOMT in lignin biosynthesis pathway and CHS and CHI in flavonoid pathway.When only TR4 was inoculation,the expression of related genes in‘Brazilian’mainly showed significant down-regulation,while it was mainly up-regulated in‘Nongke No.1’.When treated with SA only,C4H and 4CL were up-regulated in the two cultivars.After SA treatment and TR4 inoculation,the expression of C4H and 4CL in the two cultivars’maintained up-regulated trend.The up-regulation range in‘Nongke No.1”was higher compared with‘Brazilian’.The expression of CAD in‘Bralizian’was up-regulated treated with SA,while it decreased when further inoculated with TR4.The expression of CAD in‘Nongke No.1’was also up-regulated treated with SA,and so was it in‘Nongke No.1’under the double treatments with SA and TR4.CCoAOMT in‘Brazilian’was

关 键 词：香蕉枯萎病 诱导抗病性 苯丙烷类途径 基因表达 

分 类 号：S432.2.3[农业科学—植物病理学]