人参内生真菌Aspergillus terreus RSB2007的次生代谢产物的研究  

The secondary metabolites from endophytic fungus Aspergillus terreus RSB2007 from Panax ginseng

作　　者：张红 毛伟业 郑彬玥 刘霞 金璐 李圣宇 傅惠英 寿旗扬[1,2] ZHANG Hong;MAO Wei-ye;ZHENG Bin-yue;LIU Xia;JIN Lu;LI Sheng-yu;FU Hui-ying;SHOU Qi-yang(The Second Clinical Medical College&Second Affiliated Hospital,Zhejiang Chinese Medical University,Hangzhou 310005,China;Pharmacy Division of Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学附属第二医院,浙江杭州310005 [2]浙江中医药大学药学院,浙江杭州310053

出　　处：《中草药》2022年第17期5265-5270,共6页Chinese Traditional and Herbal Drugs

基　　金：浙江省自然科学基金项目(LQ22H280005)。

摘　　要：目的 研究人参内生真菌AspergillusterreusRSB2007次生代谢产物的化学成分,以期发现新的化合物。方法 该菌大米固体发酵的醋酸乙酯提取物经硅胶、SephadexLH-20及HPLC等多种色谱技术进行分离纯化,并根据核磁共振谱、质谱、圆二色谱(electroniccirculardichroism,ECD)以及计算ECD等方法鉴定化合物的结构。结果 从人参内生真菌Aspergillus terreus RSB2007发酵产物中初步分离鉴定了4个化合物,分别鉴定为7,8′-二羟基-3,4,6-三甲基-7′-苯基-1,3,4,7′-四氢-2H-吡喃并[2,3,4-de]色烯-8′-羧酸盐(1)、butyrolactone II(2)、aspernolide A(3)和versicolactone B(4)。结论 化合物1为新的聚酮类化合物,命名为曲霉酮A。Objective To study the secondary metabolites of the endophytic fungus Aspergillus terreus RSB2007 from Panax ginseng C. A. Meyer in order to obtain new compounds. Methods The EtOAc extracts of fungal rice cultures were separated and purified by using various chromatographic methods(silica gel, Sephadex LH-20 and HPLC). The structures of all compounds were determined by chemical and spectroscopic methods(NMR, HRESIMS, CD, ECD, etc.). Results Four compounds were preliminarily isolated and their structures were identified as 7,8′-dihydroxy-3,4,6-trimethyl-7′-phenyl-1,3,4,7′-tetrahydro-2Hpyrano[2,3,4-de]chromene-8′-carboxylate(1), butyrolactone II(2), aspernolide A(3) and versicolactone B(4). Conclusion Compound 1 is a new polyketide and named aspercitrinol A.

关 键 词：人参 内生真菌 次生代谢产物 聚酮 曲霉酮A 

分 类 号：R284.1[医药卫生—中药学]