产地加工炮制一体化与传统何首乌饮片化学成分的比较研究  

Comparative study on chemical components of Polygonum multiflorum between integrated processing and traditional processing

作　　者：黄孟秋 孙连娜 董志颖[1] 屠燕 邱实 HUANG Meng-qiu;SUN Lian-na;DONG Zhi-ying;TU Yan;QIU Shi(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学,上海201203

出　　处：《中草药》2022年第17期5293-5304,共12页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金青年基金项目(81903745);上海市浦江人才计划(19PJ1409300)。

摘　　要：目的 全面表征何首乌Polygonum multiflorum的化学成分,对产地加工炮制一体化何首乌饮片与传统加工何首乌饮片的化学成分进行比较。方法 采用超高效液相色谱-四极杆飞行时间串联质谱(UPLC-QTOF-MS)技术表征何首乌中的化学成分;运用代谢组学方法比较产地加工炮制一体化何首乌饮片与传统饮片中成分差异。结果 根据对照品的质谱裂解规律、保留时间、色谱峰的精确相对分子质量、碎片离子等信息结合MS-FINDER软件从何首乌中鉴定出了69个化学成分;运用多元统计分析比较产地加工炮制一体化饮片与传统饮片的化学成分,整体化学轮廓存在相似性,但大黄素、二苯乙烯苷、大黄素-8-O-β-D-葡萄糖苷等游离蒽醌类成分存在差异。结论 产地加工炮制一体化技术具有简化炮制步骤,提高生产效率等优点,但是在推广产地加工炮制一体化技术的过程中,还应考虑到游离蒽醌类成分的影响。Objective To analyze and identify the chemical components from Heshouwu(Polygonum multiflorum) and compare the differences of chemical components between integrated processed and traditional processed slices by metabolomics. Methods The chemical components of P. multiflorum were characterized by ultra performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-QTOF-MS), and the metabolomics approach was used to compare the differences of components between integrated processed and traditional processed slices. Results A total of 69 components were identified from P.multiflorum by MS-FINDER software based on the information of the reference standards, accurate molecular weight of chromatographic peaks, fragmentation patterns and other information. Multivariate statistical analysis was applied to compare the differences of chemical components between the integrated pieces and the traditional slices. The results showed that no significant differences were observed in the overall chemical profile, whereas there were differences in free anthraquinones such as emodin,stilbene glucoside and emodin-8-O-β-D-glucoside. Conclusion The integrated technology of origin producing and processing is more advantageous than the traditional processing in simplifying processing steps and improving production efficiency. However, in the process of promoting the integrated processing method, the influence of free anthraquinones should be considered.

关 键 词：何首乌 产地加工炮制一体化 超高效液相色谱-四极杆飞行时间串联质谱 化学成分 代谢组学 大黄素 二苯乙烯苷 大黄素-8-O-β-D-葡萄糖苷 

分 类 号：R284.1[医药卫生—中药学]