A comparative study on the genomes,transcriptomes,and metabolic properties of Escherichia coli strains Nissle 1917,BL21(DE3),and MG1655  

作　　者：Linlin Zhao Guobin Yin Yonglin Zhang Chaofan Duan Yang Wang Zhen Kang 

机构地区：[1]The Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China [2]The Science Center for Future Foods,Jiangnan University,Wuxi 214122,China [3]The Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China

出　　处：《Engineering Microbiology》2022年第1期8-16,共9页工程微生物学（英文）

基　　金：supported by the National Key Re-search and Development Program of China(2021YFC2100800);the Jiangsu Province Natural Science Fund for Distinguished Young Schol-ars(BK20200025);a grant from the Key Technologies R&D Program of Jiangsu Province(BE2019630);the National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-16).

摘　　要：Escherichia coli is the most well-studied model prokaryote and has become an indispensable host for the biotech-nological production of proteins and biochemicals.In particular,the probiotic status of one E.coli strain,E.coli Nissle 1917(EcN)has helped it become a new favorite amongst synthetic biologists.To broaden its potential applications,here we assemble a comparative study on the genomes,transcriptomes,and metabolic properties of E.coli strains EcN,BL21(DE3),and MG1655.Comparative genomics data suggests that EcN possesses 1404 unique CDSs.In particular,EcN has additional iron transport systems which endow EcN with a higher tolerance to iron scarcity when compared to two other E.coli strains.EcN transcriptome data demonstrates that E.coli strains EcN,BL21(DE3),and MG1655 all have comparable activities of the central metabolic pathway,however only EcN inherits the arginine deiminase pathway.Additionally,we found that EcN displayed a lower expres-sion of ribosomal proteins compared to BL21(DE3)and MG1655.This comparative study on E.coli strains EcN,BL21(DE3),and MG1655 aims to provide a reference for further engineering EcN as a biotechnological tool.

关 键 词：Escherichia coli Nissle 1917 PROBIOTICS comparative genomics TRANSCRIPTOME synthetic biology 

分 类 号：Q78[生物学—分子生物学]