Multiscattering-enhanced absorbance to enable the sensitive analysis of extremely diluted biological samples: Determination of oxidative potential in exhaled air  

作　　者：Sami Goekce Nicolas Concha-Lozano Jean-Jacques Sauvain Maud Hemmendinger Alejandro Portela Elise Sergent Pascal Andujar Jean-Claude Pairon Pascal Wild Guillaume Suarez 

机构地区：[1]Center for Primary Care and Public Health,Lausanne University,Lausanne,Switzerland [2]Forensic Toxicology and Chemistry Unit,CURML,Lausanne University Hospital,Geneva University Hospitals,Switzerland [3]International University of Catalonia,Bioengineering Institute of Technology,Barcelona,Spain [4]CHI Creteil,Service Pathologies Professionnelles et de L’Environnement,Creteil,France [5]Universite Paris-Est Creteil,Faculte de Sante,Institut Sante Travail Paris-Est Creteil,France [6]Inserm U955,Team 4,Creteil,France [7]INRS,Scientific Management,Vandoeuvre Les Nancy,France

出　　处：《Medicine in Novel Technology and Devices》2022年第2期62-66,共5页医学中新技术与新装备（英文）

基　　金：This study was supported in the frame of the project OxAirDirect(Grant EST2015-1-151)funded by the French Agency for Food,Environmental and Occupational Health&Safety(ANSES);the project ROBoCoP(Grant IZCOZ0-177,067)funded by the Swiss National Science Foundation(SNSF);the framework of CliniMARK COST Action(CA16113).

摘　　要：Direct determination of the oxidative potential in exhaled air(OPEA)as a biological indicator of the redox balance status in airways requires an extremely sensitive sensing approach to counterbalance the high dilution of the exhaled air matrix.In opposite to standard colorimetric assays based on Beer-Lambert Law,the use of simple multiscattering-enhanced absorbance(MEA)strategy results in an improved photonic sensing system.Based on this strategy and using the ferrous-xylenol orange assay,analytical performances allowing the detection of 3 pmol of H_(2)O_(2) could be obtained.The comparative analysis of photometric configurations–standard vs MEA–highlights the large absorbance gain obtained for tiny H_(2)O_(2) amounts–from 0 to 30 pmol–whereas similar sensing trends are observed at higher concentration range.The automated introduction of exhaled air sample into the reaction compartment via needle-impinger coupled with the MEA-based photonic cell enables ultrasensitive determination of OPEA in short time(<5 min).A pilot study conducted on healthy subjects(n=22)and chronic obstructive pulmonary disease(COPD)patients(n=23)volunteers shows a significant increase of the OPEA for COPD versus controls and strong negative correlation between OPEA and spirometry parameters(Tiffeneau index:FEV1/FVC)for ex-/non-smokers.Application perspectives to assess inflammatory episodes in obstructive lung diseases are thus made possible using this sensing approach.

关 键 词：Absorbance enhancement Photonic device Oxidative potential Exhaled air COPD Pilot study 

分 类 号：O657.3[理学—分析化学]