毛蕊异黄酮干预Hippo通路抑制三阴性乳腺癌MDA-MB-231细胞增殖与迁移的机制  

Calycosin inhibits proliferation and migration of human triple-negative breast cancer cells through intervening Hippo pathway

作　　者：孙腾飞 李俊峰[1] 陶方方[1] 赵俊慧 刘青玲 刘文洪[1] SUN Teng-fei;LI Jun-feng;TAO Fang-fang;ZHAO Jun-hui;LIU Qing-ling;LIU Wen-hong(School of Basic Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学基础医学院,浙江杭州310053

出　　处：《中国药理学通报》2022年第10期1517-1523,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 82074391);浙江省基础公益研究计划项目(No LGN20H280002)。

摘　　要：目的研究毛蕊异黄酮(calycosin,CA)对人三阴性乳腺癌MDA-MB-231细胞增殖、迁移的影响及其作用机制。方法CA干预MDA-MB-231细胞,采用CCK-8法检测细胞增殖能力;流式细胞术检测MDA-MB-231细胞凋亡和周期;细胞划痕观察CA对MDA-MB-231细胞迁移的影响;RT-PCR检测细胞上皮间质转化(epithelial-mesenchymal transition,EMT)相关基因mRNA表达水平;运用Western blot法检测CA对Hippo通路关键蛋白及EMT相关蛋白表达的影响。结果CA可明显抑制MDA-MB-231细胞的增殖、迁移,促进凋亡,抑制Hippo信号通路和EMT相关蛋白表达水平。结论CA可能通过抑制Hippo信号通路和EMT途径诱导MDA-MB-231细胞凋亡、阻滞细胞周期、抑制细胞迁移。Aim To study the effects of calycosin on proliferation and migration of human triple-negative breast cancer MDA-MB-231 cells and the underlying mechanisms.Methods MDA-MB-231 cells were intervened by calycosin,and the proliferation ability was detected by CCK-8 method.The apoptosis and cycle of MDA-MB-231 cells were detected by flow cytometry.The effect of calycosin on the migration of MDA-MB-231 cells was observed using cell scratch.The mRNA expression of EMT related genes was detected by RT-PCR.The effects of calycosin on the expression of key proteins of Hippo pathway and EMT related proteins were detected by Western blot.Results Calycosin could significantly inhibit the proliferation,migration and apoptosis of MDA-MB-231 cells,and markedly inhibit the expression of Hippo signaling pathway and EMT related protein.Conclusion Calycosin may induce MDA-MB-231 cell apoptosis,block cell cycle and inhibit cell migration by inhibiting Hippo signaling pathway and EMT pathway.

关 键 词：毛蕊异黄酮 三阴性乳腺癌 细胞周期 Hippo通路 迁移 增殖 

分 类 号：R284.1[医药卫生—中药学] R329.28[医药卫生—中医学] R737.9