运用BioID技术筛选S100A7互作蛋白  

Screening of S100A7 Interacting Proteins Using BioID Technology

作　　者：王俊豪 孔菲 李允广 刘进[1] 胡恩泽 王睿 黄凌云[1] 肖雪媛[1] WANG Jun-hao;KONG Fei;LI Yun-guang;LIU Jin;HU En-ze;WANG Rui;HUANG Ling-yun**();XIAO Xue-yuan**()(Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education,College of Life Sciences,Beijing Normal University,Beijing 100875,China)

机构地区：[1]北京师范大学生命科学学院细胞增殖及调控生物学教育部重点实验室,北京100875

出　　处：《中国生物工程杂志》2022年第8期52-62,共11页China Biotechnology

基　　金：国家重点基础研究计划(2011CB915504);国家自然科学基金(81172216)资助项目。

摘　　要：目的:通过邻近生物素鉴定(BioID)技术筛选S100A7互作蛋白并进行验证。方法:采用邻近生物素BioID和质谱相结合的方法,筛选S100A7互作蛋白。通过免疫荧光和免疫共沉淀方法,对相互作蛋白进行验证。结果:与对照组比对,通过BioID技术共获得94个可能与S100A7相互作用的候选蛋白质。选取Annexin A2(AnxA2)蛋白在HEK293细胞中进行了验证,结果表明S100A7与AnxA2存在直接的相互作用,且二者共定位于细胞质和细胞膜。结论:可将BioID技术作为互作蛋白筛选的一项新技术,通过该技术发现S100A7和AnxA2存在相互作用。Objective:To investigate and verify the candidate interactors of S100 A7.Methods:The candidate interactors of S100 A7 are identified using BioID and mass spectrum and verified by Co-IP and immunofluorescence.Results:94 candidate proteins are identified in two groups by mass spectrum.Interaction between Annexin A2(AnxA2)and S100 A7 was then verified using Co-IP in HEK293 WT cells.It is demonstrated that AnxA2 interacts with S100 A7 directly,and the immunofluorescence results show that S100 A7 and AnxA2 are collocating in the cytoplasm.Conclusion:BioID is a new technology for screening S100 A7 interactors and the interaction between S100 A7 and AnxA2 is discovered using BioID.

关 键 词：S100A7 BioID Annexin A2 互作蛋白 

分 类 号：Q819[生物学—生物工程]