臻通集胶囊对高脂血症模型大鼠的治疗作用及其机制研究  

Study on the Treatment and Mechanism of Zhentongji Capsule(臻通集胶囊)on Hyperlipidemia Model Rats

作　　者：侯红平[1] 王彩霞[1] 彭博[1] 陈腾飞 赵晓晓 宋玲 高云航 练东银 李丽丽 张广平[1] HOU Hongping;WANG Caixia;PENG Bo;CHEN Tengfei;ZHAO Xiaoxiao;SONG Ling;GAO Yunhang;LIAN Dongyin;LI Lili;ZHANG Guangping(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Hebei Yuzhilin Biotech Co.,Ltd.,Shijiazhuang Hebei 050000,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]河北御芝林生物科技有限公司,河北石家庄050000

出　　处：《中医药导报》2022年第9期14-18,24,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：中央级公益性科研院所基本科研业务费专项基金资助项目(ZZ13-YQ-060,ZXKT21020)。

摘　　要：目的:探讨臻通集胶囊对高脂血症模型大鼠的疗效及其作用机制。方法:随机取12只SD大鼠作为空白组,正常饮食;取88只大鼠进行造模,给予高脂饲料喂养4周后,测定TG和CHO。取造模成功的72只大鼠根据CHO值随机分为模型组、西药组、中西药联合组、中药高剂量组、中药中剂量组、中药低剂量组。各药物组大鼠灌胃给予相应药物,空白组和模型组大鼠灌胃给予等体积的生理盐水,1次/d,连续给药8周后,麻醉采血,称体质量和肝脏质量,计算肝脏系数;检测血清中血脂水平TG、CHO、LDL、HDL和TBA的表达水平,并计算H/L值;检测载脂蛋白Apo-A1和Apo-B100的表达水平;检测MDA、SOD和T-AOC等氧化应激水平,同时检测肝脏组织中胆固醇合成相关酶HMG-COA和ACAT的表达;HE染色进行病理观察。结果:与空白组比较,模型组大鼠体质量及肝脏系数,血清中CHO、TG、LDL、HDL、Apo-A1、Apo-B100、MDA含量,肝脏组织中HMG-COA、ACAT含量均明显升高(P<0.05),而血清中SOD、T-AOC活性及H/L均明显降低(P<0.05);与模型组比较,西药组、中西药联合组和中药高剂量组大鼠体质量及肝脏系数均明显降低(P<0.05),中药中剂量组大鼠肝脏系数明显降低(P<0.05);与模型组比较,西药组、中西药联合组、中药高剂量组、中药中剂量组大鼠血清CHO、TG、LDL含量均明显降低(P<0.05),H/L均明显升高(P<0.05);中西药联合组、中药高剂量组大鼠血清HDL含量均明显低于模型组(P<0.05);与模型组比较,中西药联合组、中药高剂量组大鼠血清中Apo-A1、Apo-B100含量均明显降低(P<0.05),西药组和中药低剂量组Apo-B100显著降低(P<0.05);与模型组比较,中西药联合组、中药中剂量组、中药高剂量组大鼠血清中MDA含量明显降低(P<0.05),而SOD、T-AOC活性明显升高(P<0.05);与模型组比较,中西药联合组、中药高剂量组大鼠肝脏组织中HMG-COA、ACAT含量均明显降低(P<0.05);西药组大鼠肝脏组织�Objective:To explore the curative effect of Zhentongji Capsule on hyperlipemia model rats and its preliminary mechanism.Methods:12 SD rats were randomly selected as the blank group with normal diet.88 rats were fed with high-fat diet for 4 weeks,and TG and CHO were measured.72 successful rats were divided into model group,western medicine group,combined Chinese and western medicine group,Chinese medicine high,middle and low dose group according to CHO value according to CHO value.Rats in each drug group were given corresponding drugs by gavage.Rats in the blank group and model group were given the same volume of normal saline by gavage,once a day.After 8 weeks of continuous administration,blood was collected under anesthesia,body mass and liver mass were weighed,and liver coefficient was calculated.The expression levels of lipid level TG,CHO,LDL,HDL and TBA in serum were detected,and the H/L value was calculated.The expression levels of apolipoprotein Apo-A1 and Apo-B100 were detected.The oxidative stress levels of MDA,SOD,T-AOC were detected,and the expression of cholesterol synthesis related enzymes HMG-COA and ACAT in liver tissue was detected.He staining was used for pathological observation.Results:Compared with the blank group,the body mass and liver coefficient,the contents of CHO,TG,LDL,HDL,Apo-A1,Apo-B100 and MDA in the serum,the contents of HMG-COA and ACAT in the liver tissue of the model group were significantly increased(P<0.05),while the activities of SOD,T-AOC and H/L in the serum were significantly decreased(P<0.05).Compared with the model group,the body mass and liver coefficient of rats in the western medicine group,the combined Chinese and western medicine group and the high-dose Chinese medicine group were significantly decreased(P<0.05),and the liver coefficient of rats in the middle dose Chinese medicine group was significantly decreased(P<0.05).Compared with the model group,the serum CHO,TG and LDL contents of rats in the western medicine group,the combined Chinese and western medicine grou

关 键 词：高脂血症 臻通集胶囊 血脂 胆固醇 氧化应激 大鼠 

分 类 号：R285.5[医药卫生—中药学]