‘兰引3号’结缕草足球场丛枝菌根真菌多样性及菌群结构分析  

Diversity and community structure analysis of arbuscular mycorrhizal fungi in Zoysia japonica‘Lanyin No.3’football fields

作　　者：张楚婷 彭雪 宋玉 辛国荣[1] ZHANG Chu-ting;PENG Xue;SONG Yu;XIN Guo-rong(State Key Laboratory of Biocontrol,Guangdong Provincial Key Laboratory of Plant Resources,School of Agriculture,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]有害生物控制与资源利用国家重点实验室,广东省热带亚热带植物资源重点实验室,中山大学农学院,广东广州510275

出　　处：《草原与草坪》2022年第3期27-34,44,共9页Grassland and Turf

基　　金：国家自然科学基金(31071357)。

摘　　要：为筛选合适的AMF菌剂,以解决华南地区常见的‘兰引3号’结缕草(Zoysia japonica‘Lanyin No.3’)足球场草坪养护管理过程中存在的水肥浪费和潜在的环境问题。本研究运用扩增子技术对4个以‘兰引3号’结缕草建植的足球场草坪AMF进行群落结构和物种鉴定。结果表明,4个足球场的‘兰引3号’结缕草均与AMF形成了共生结构,侵染形式以菌丝侵染为主。通过多样性分析发现,在管理强度较低的英东体育场(YD)中,‘兰引3号’结缕草根际土壤和根内AMF多样性均高于其他管理强度较高的场地。草坪中的AMF大部分属于球囊霉属(Glomus),其次为盾巨孢囊霉属(Scutel⁃lospora)以及无梗囊霉属(Acaulospora);可见,球囊霉属真菌是组成高效AMF菌剂的重要菌种资源,能为‘兰引3号’结缕草足球场草坪保水、保肥等菌根技术的发展提供保障。Arbuscular mycorrhizal fungi(AMF)can form mutualistic symbiosis with plant roots,which is beneficial to plant growth and development.In order to screen the AMF suitable for Zoysia turf,amplicon technique was used in the molecular identification of the AMF community structure and species of four football field turf plants with Zoysia japonica‘Lanyin No.3’.The result showed that Z.japonica‘Lanyin No.3’in all football fields formed sym‐biotic structures with AMF,and the main form of infection was hyphal infection.The AMF communities in all the lawns were dominated by Glomus,followed by Scutellospora and Acaulospora.In conclusion,the AMF inoculants with the Glomus fungus as the main component could provide guarantee for the constrution and development of mycorrhizal technologies such as water and fertilizer conservation of Z.japonica‘Lanyin No.3’football lawn.

关 键 词：‘兰引3号’结缕草 AMF菌剂 扩增子测序 群落结构 足球场草坪 

分 类 号：S688.4[农业科学—观赏园艺]