外源2,3-丁二醇对匍匐翦股颖耐热性的影响  

Preliminary study on improving heat resistance of creeping bentgrass by exogenous 2,3-butaneglycol

作　　者：赵宁 史毅 马晖玲[1] ZHAO Ning;SHI Yi;MA Hui-ling(College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem,Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grassland Ecosystem Sustainability,Lanzhou 730070,China;Gansu Agricultural University College of Forestry,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中‐美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃农业大学林学院,甘肃兰州730070

出　　处：《草原与草坪》2022年第3期54-62,共9页Grassland and Turf

基　　金：甘肃农业大学学科建设基金“植物根际益生菌挥发性化合物类似物诱导匍匐翦股颖耐热性机理研究”(GAU-XKJS-2018-107);甘肃省高等学校创新基金项目“2,3-丁二醇调控热胁迫下匍匐翦股颖绿期及植株发育的研究”(2021B-133)。

摘　　要：针对匍匐剪股颖(Agrostis stolonifera)在北过渡带不能安全越夏的问题,本试验模拟设置人工气候热胁迫(37℃/32℃,昼/夜),通过喷施300μmol/L 2,3-丁二醇(2,3‐butanediol,BD),测定分析匍匐翦股颖Penn A4幼苗在热胁迫第0、7、14、21、28天时叶片光合色素、细胞膜损伤程度、抗氧化酶活及渗透调节物质含量的变化,研究外源BD对Penn A4耐热性的影响。结果表明:常温环境下,经BD处理的Penn A4各项生理指标较对照组无显著差异(P>0.05);高温胁迫下,随着胁迫时间的延长,BD处理显著减缓了Penn A4叶片光合色素的降解速度(P<0.05),有效抑制了叶片电解质外渗,降低了丙二醛和过氧化氢含量。与高温对照相比,外源BD提高了热胁迫下(≤14 d)Penn A4植株的超氧化物歧化酶、过氧化物酶、抗坏血酸过氧化物酶和谷胱甘肽还原酶活性,促进了游离脯氨酸和可溶性蛋白含量的积累。叶面喷施BD通过提高胁迫前期Penn A4植株的抗氧化酶活性和渗透调节物质含量,维持了细胞膜的稳定性和活性氧的代谢平衡,缓解了高温造成的氧化损伤,提高了匍匐剪股颖耐热性。In order to solve the problem that the creeping bentgrass(Agrostis stolonifera)could not survive the summer safely in the north transition zone,this experiment simulated the heat stress of artificial climate(37℃/32℃,day/night).By spraying 300μmol/L 2,3‐butanediol(BD),the changes of leaf photosynthetic pigment,cell membrane damage degree,antioxidant enzyme activity and osmotic regulation substance content in Penn A4 seedlings of creeping bentgrass at 0,7,14,21 and 28 days under heat stress were determined and analyzed.The effect of exogenous BD on heat resistance of Penn A4 was studied in this way.The results showed that under normal temperature environment,physiological indexes of Penn A4 treated with BD showed no significant difference compared with the control group(P>0.05).The results also implied under high temperature stress,BD treatment significantly slowed down the degradation rate of photosynthetic pigments in Penn A4 leaves(P<0.05),effectively inhibited leaf electrolyte exosmotic,and reduced the contents of malondialdehyde and hydrogen peroxide with the extension of stress time.Compared with the high temperature control,exogenous BD increased the activities of superoxide dismutase,peroxidase,ascorbate peroxidase and glutathione reductase of Penn A4 plants under heat stress(≤14 d),and promoted the accumulation of free proline and soluble protein content.In short,foliar spraying of BD could increase the antioxidant enzyme activity and osmotic adjustment substance content of Penn A4 plants in the early stage of stress,maintain the stability of cell membrane and the metabolic balance of reactive oxygen species,thereby alleviating the oxidative damage caused by high temperature and improving the plant heat resistance.

关 键 词：2 3-丁二醇 高温胁迫 匍匐翦股颖 抗氧化系统 细胞膜系统 耐热性 

分 类 号：S688.4[农业科学—观赏园艺]