The Arabidopsis spliceosomal protein SmEb modulates ABA responses by maintaining proper alternative splicing of HAB1  

作　　者：Yechun Hong Juanjuan Yao Huazhong Shi Yunjuan Chen Jian-Kang Zhu Zhen Wang 

机构地区：[1]Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,200032 Shanghai,China [2]University of Chinese Academy of Sciences(CAS),Shanghai 200032,China [3]Department of Chemistry and Biochemistry,Texas Tech University,Lubbock,TX 79409,USA

出　　处：《Stress Biology》2021年第1期38-45,共8页逆境生物学（英文）

基　　金：supported by the National Natural Science Foundation of China(grant 32000206 to Z.W.);the Youth Innovation Promotion Association(2020273 to Z.W.)of the Chinese Academy of Sciences.

摘　　要：Abscisic acid(ABA)signaling is critical for seed germination and abiotic stress responses in terrestrial plants.PremRNA splicing is known to regulate ABA signaling.However,the involvement of canonical spliceosomal components in regulating ABA signaling is poorly understood.Here,we show that the spliceosome component Sm core protein SmEb plays an important role in ABA signaling.SmEb expression is up-regulated by ABA treatment,and analysis of Arabidopsis smeb mutant plants suggest that SmEb modulates the alternative splicing of the ABA signaling component HAB1 by enhancing the HAB1.1 splicing variant while repressing HAB1.2.Overexpression of HAB1.1 but not HAB1.2 rescues the ABA-hypersensitive phenotype of smeb mutants.Mutations in the transcription factor ABI3,4,or 5 also reduce the ABA hypersensitivity of smeb mutants during seed germination.Our results show that the spliceosomal component SmEb plays an important role in ABA regulation of seed germination and early seedling development.

关 键 词：ABA signaling Alternative splicing Cotyledon greening HAB1 SmEb 

分 类 号：Q94[生物学—植物学]