Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca^(2+)  

作　　者：Qiao Bai Xiaoping Feng Yu Wang Chunyu Wu Ye Liu Jiao Sun Tianli Yue Fangyu Long 

机构地区：[1]College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China [2]Beijing Key Laboratory of Plant Resource Research and Development,Beijing Technology and Business University,Beijing 100048,China [3]Department of Breast Surgery,The Affi liated Hospital of Qingdao University,Qingdao 266000,China

出　　处：《Food Science and Human Wellness》2023年第3期832-841,共10页食品科学与人类健康（英文）

基　　金：supported by grant from the National Key Research and Development Program of China(2018YFC1602201);the Open Research Fund Program of Beijing Key Lab of Plant Resource Research and Development,Beijing Technology and Business University(PRRD-2021-YB8);the National Natural Science Fund(31601395);the Key Program for Shaanxi Science and Technology(2020NY-146)。

摘　　要：Jujube contains abundant cyclic adenosine monophosphate(cAMP)and the ultrasonic-assisted pectinase extraction(UAPE)conditions for obtaining the maximum cAMP yield from jujube were optimized.Orthogonal array design was applied to evaluate the effects of 4 variables by UAPE on cAMP yield.The results showed that the optimal cAMP yield(783.0μg/g)was derived at ratio of liquid to solid 5 mL/g,ratio of pectinase to raw material 1.5%,time 60 min and temperature 40℃.Moreover,the effect of cAMP on the anti-allergic function of action induced by immunoglobulin E(IgE)and its meschanism was investigated through establishing the sensitized cell model in rat basophilic leukemia(RBL-2 H3)cells using dinitrophenylated(DNP)-bovine serum albumin(BSA)-IgE.The results showed that cAMP interfered with sensitized cells,effectively inhibited the occurrence of basophil degranulation in dose dependence,and significantly reduced the activity ofβ-hexosamindase(β-hex),at the optimal concentration of 50μg/mL.The level of anti-inflammatory factor interleukin-10(IL-10)was promoted and the content of pro-inflammatory factor tumor necrosis factor-α(TNF-α)was suppressed by cAMP.In addition,influx of intracellular Ca^(2+) was repressed effectively.Our results demonstrate that jujube cAMP regulated the cytokine balance in the allergy pathway through blocking the influx of extracellular Ca^(2+),with the prevention of allergy symptoms.

关 键 词：Ultrasonic-assisted pectinase extraction Cyclic adenosine monophosphate Rat basophilic leukemia cells Ca^(2+)influx Allergy 

分 类 号：R285.5[医药卫生—中药学]