基于全基因组重测序及等位基因频率解析柔嫩艾美耳球虫对地克珠利耐药分子机制的初步研究  

Preliminary Study of Molecular Mechanisms of Diclazuril Resistance in Eimeria tenella Based on Whole-Genome Resequencing and Allele Frequency Analysis

作　　者：毕菲菲 郝振凯 孙霈 于咏兰[1] 刘贤勇 索勋[1] BI Fei-fei;HAO Zhen-kai;SUN Pei;YU Yong-lan;LIU Xian-yong;SUO Xun(College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学动物医学院,北京海淀100193

出　　处：《中国兽医杂志》2022年第8期1-6,共6页Chinese Journal of Veterinary Medicine

基　　金：国家重点研发计划(2018YFD0500300,2016YFD0501303);国家自然科学基金(31873007)。

摘　　要：鸡球虫病是由艾美耳球虫感染引起的一种肠道内寄生虫病。地克珠利作为常见的抗球虫药物,其长期使用导致具有耐药性的鸡球虫广泛出现。为解析鸡球虫对地克珠利产生耐药性的分子机制,本研究从田间收集了1株柔嫩艾美耳球虫(Eimeria tenella)地克珠利耐药株与3株柔嫩艾美耳球虫地克珠利敏感株,并进一步对收集到的4株田间株进行了全基因组重测序和等位基因频率(ΔSNP-index)分析。结果显示,柔嫩艾美耳球虫基因组中表面抗原(SAG)基因(EVM0000025)存在1个位点的非同义突变(D12E),此突变可能与地克珠利耐药性的产生相关。本研究为解析鸡球虫对地克珠利产生耐药性的分子机制提供了科学依据。Chicken coccidiosis is an intestinal parasitic disease caused by Eimeria infection.Diclazuril is a commonly used anticoccidial drug but drug-resistant coccidia emerged after its long-term application in chickens.In order to analyze the molecular mechanism of the drug resistance of chicken coccidia to diclazuril,1 diclazuril-resistant and 3 diclazuril-sensitive strains of Eimeria tenella were isolated from the field and examined by whole-genome resequencing and allele frequency(ΔSNP-index)analysis.The results indicated that non-synonymous mutations(D12E)at a site in the surface antigen gene(EVM0000025)of E.tenella may underscore the emergence of diclazuril resistance.This study provides a scientific basis for analyzing the molecular mechanism of chicken coccidia resistance to diclazuril.

关 键 词：地克珠利 鸡球虫 ΔSNP-index 耐药性 分子机制 

分 类 号：S858.31[农业科学—临床兽医学]