外胚间充质干细胞条件培养基冻干粉复合纤维蛋白胶修复大鼠皮肤损伤  

Ecto-mesenchymal stem cell-conditioned medium lyophilized powder combined with fibrin glue to repair skin injury in rats

作　　者：陈頔 薛钰 汤郁[1] 费小明[3] 庄琴 周雯雯 吕德民 史文涛 张志坚[2] 郑文娟 蒋宇 Chen Di;Xue Yu;Tang Yu;Fei Xiaoming;Zhuang Qin;Zhou Wenwen;Lyu Demin;Shi Wentao;Zhang Zhijian;Zheng Wenjuan;Jiang Yu(Department of Rheumatology and Immunology,Affiliated Hospital of Jiangsu University,Zhenjiang 212001,Jiangsu Province,China;Medical School,Jiangsu University,Zhenjiang 212000,Jiangsu Province,China;Department of Hematology,Affiliated Hospital of Jiangsu University,Zhenjiang 212001,Jiangsu Province,China;Department of General Practice,Affiliated Hospital of Jiangsu University,Zhenjiang 212001,Jiangsu Province,China)

机构地区：[1]江苏大学附属医院风湿免疫科,江苏省镇江市212001 [2]江苏大学附属医院,江苏省镇江市212001 [3]江苏大学附属医院全科,江苏省镇江市212001 [4]江苏大学医学院,江苏省镇江市212000

出　　处：《中国组织工程研究》2023年第15期2350-2355,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81571582),项目负责人:汤郁;江苏省社会发展重点项目-临床先进技术(BE2020681),项目负责人:费小明。

摘　　要：背景:近来多数研究将组织工程材料与干细胞或细胞因子复合来改善动物模型皮肤损伤。鼻黏膜来源的外胚间充质干细胞条件培养基冻干粉可以长期保存,用于修复皮肤损伤具有独特的优势。目的:观察外胚间充质干细胞条件培养基冻干粉复合纤维蛋白胶修复大鼠皮肤缺损的效果。方法:体外培养SD大鼠鼻黏膜来源的外胚间充质干细胞,第3代细胞的培养基透析后冷冻干燥制成冻干粉。将30只皮肤缺损SD大鼠按治疗方法分为3组(n=10):损伤对照组,不接受特殊治疗作为对照;纤维蛋白胶组,局部应用纤维蛋白胶;条件培养基冻干粉复合纤维蛋白胶组,局部应用含有外胚间充质干细胞条件培养基冻干粉的纤维蛋白胶。治疗后动态观察创面愈合情况,测量创面面积,对皮肤组织切片进行苏木精-伊红染色和CK8、P63免疫组化染色。结果与结论:条件培养基冻干粉复合纤维蛋白胶组创面愈合速度高于纤维蛋白胶组和损伤对照组(P<0.001)。条件培养基冻干粉复合纤维蛋白胶组创面表皮和真皮在21 d全部再生修复;纤维蛋白胶组再生皮肤较薄;单纯创伤组皮肤再生不完全。在大鼠模型中,外胚间充质干细胞条件培养基冻干粉复合纤维蛋白胶联合使用可以促进损伤皮肤的伤口愈合。BACKGROUND:Recently,most studies have combined tissue engineering materials with stem cells or factors to improve animal skin injury models.Ecto-mesenchymal stem cells derived from nasal mucosa can be preserved for a long time and has unique advantages in repairing skin damage.OBJECTIVE:To observe the effect of ecto-mesenchymal stem cells-conditioned medium lyophilized powder combined with fibrin glue on the wound healing of skin defects in rats.METHODS:Ecto-mesenchymal stem cells derived from Sprague-Dawley rat nasal mucosa were cultured in vitro,and the conditioned medium from passage 3 was collected and lyophilized.The 30 rat models of skin defects were divided into three groups according to treatment(n=10):the injury control group,received no specific treatment as control;the fibrin glue group,received topical application of fibrin glue;the combination group,received topical application of the fibrin glue contained ecto-mesenchymal stem cells-conditioned medium lyophilized powder.After treatments,the wound healing was observed dynamically and the wound area was measured.The tissue sections of the skin were stained with hematoxylin and eosin and subjected to immunohistochemical staining of cytokeratin-8 and protein-63.RESULTS AND CONCLUSION:The wound healing rate of the combination group was higher than that of the fibrin glue group and the injury control group(P<0.001).The staining of tissue sections showed that the epidermis and dermis of the combination group were all regenerated and repaired in 21 days;the regenerated skin in the fibrin glue group was thinner and the skin healing in the injury control group was incomplete.Ecto-mesenchymal stem cells-conditioned medium lyophilized powder in combination with fibrin glue can enhance wound healing of the injured skin in rat models.

关 键 词：皮肤损伤 鼻黏膜 外胚间充质干细胞 条件培养基 纤维蛋白胶 伤口愈合 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R622