转化生长因子β诱导骨髓间充质干细胞分化为半月板纤维软骨细胞  

Differentiation of bone marrow mesenchymal stem cells into meniscus fibrochondrocytes induced by transforming growth factor beta

作　　者：杨均 李澎[1] Yang Jun;Li Peng(Department of Joint and Sports Medicine,The Second Hospital of Dalian Medical University,Dalian 116023,Liaoning Province,China)

机构地区：[1]大连医科大学附属第二医院关节与运动医学科,辽宁省大连市116023

出　　处：《中国组织工程研究》2023年第15期2412-2419,共8页Chinese Journal of Tissue Engineering Research

基　　金：大医二院-中科院大连化物所“个体化诊疗协同创新中心”联合基金项目(UF-ZD-202013),项目负责人:李澎;大连医科大学附属第二医院临床能力提升“1+X”计划交叉学科创新项目(2022JCXKYB18),项目负责人:李澎。

摘　　要：背景:骨髓间充质干细胞可被诱导分化为纤维软骨细胞作为组织工程中的种子细胞,而细胞因子转化生长因子β(transforming growth factor beta,TGF-β1)和TGF-β3在诱导分化中的作用,目前仍是研究热点。目的:综述TGF-β1和TGF-β3诱导骨髓间充质干细胞分化为软骨细胞的相关文献,为进一步指导TGF-β诱导骨髓间充质干细胞分化为纤维软骨细胞提供理论依据,同时为今后的临床上半月板损伤和退化的治疗提供新策略。方法:计算机检索PubMed、中国知网和万方医学网数据库1990年1月至2022年3月收录的文献。英文数据库检索词:“Transforming Growth Factor beta 1,Transforming Growth Factor beta 3,Bone Marrow Mesenchymal Stem Cells,meniscus,fibrochondrocyte”,中文数据库检索词:“TGF-β1,TGF-β3、骨髓间充质干细胞、半月板、纤维软骨细胞”,根据筛选标准,最终纳入78篇文献进行综述。结果与结论:①TGF-β主要分为TGF-β1、TGF-β2和TGF-β3共3种亚型,其中研究较多的细胞因子是TGF-β1和TGF-β3。②TGF-β主要通过TGF-β/Smad信号通路激活细胞核内的SOX9转录因子进而诱导骨髓间充质干细胞分化为软骨细胞。③TGF-β1可诱导骨髓间充质干细胞向透明软骨细胞分化,伴随培养时间的延长,透明软骨细胞可发生去分化变成纤维软骨细胞;④TGF-β3主要诱导骨髓间充质干细胞向纤维软骨细胞分化,同时促进Ⅰ型胶原和蛋白聚糖合成分泌。⑤目前,关于半月板损伤和退化的治疗,临床上采用的治疗方案均不理想,组织工程的出现为其治疗提供了新的策略,具体方法是将诱导因子和骨髓间充质干细胞植入到半月板损伤部位,可以起到恢复半月板功能及再生纤维软骨组织的作用,但是目前实验尚处于体外和动物模型阶段,其在临床上用于半月板受损的治疗和可用性仍需要后续大量的临床研究去研究探索。BACKGROUND:Bone marrow mesenchymal stem cells can be induced to differentiate into fibrochondrocytes as seed cells in tissue engineering,and the application of cytokines transforming growth factor beta 1(TGF-β1)and transforming growth factor beta 3(TGF-β3)in inducing differentiation is still a research hotspot at present.OBJECTIVE:To review the literature on the differentiation of bone marrow mesenchymal stem cells into chondrocytes induced by TGF-β1 and TGF-β3,and provide a theoretical basis for further guiding the differentiation of bone marrow mesenchymal stem cells into fibrochondrocytes,as well as a new strategy for the treatment of meniscus injury and degeneration in clinic in the future.METHODS:Retrieval of PubMed database,CNKI database and Wanfang database from January 1990 to March 2022 was performed.The Chinese and English key words were“transforming growth factor beta 1;transforming growth factor beta 3;bone marrow mesenchymal stem cells;meniscus;fibrochondrocyte”.According to the screening criteria,78 articles were finally included and reviewed.RESULTS AND CONCLUSION:(1)TGF-βis mainly divided into TGF-β1,TGF-β2 and TGF-β3 subtypes,among which the most studied cytokines are TGF-β1 and TGF-β3.(2)TGF-βinduces the differentiation of bone marrow mesenchymal stem cells into chondrocytes by activating SOX9 transcription factors in the nucleus mainly through TGF-β/Smad signaling pathway.(3)TGF-β1 can induce the differentiation of bone marrow mesenchymal stem cells into hyalchondrocytes.With the extension of culture time,hyalchondrocytes can undergo dedifferentiation and become fibrochondrocytes.(4)TGF-β3 mainly induces the differentiation of bone marrow mesenchymal stem cells into fibrochondrocytes and promotes the synthesis and secretion of type I collagen and proteoglycan.(5)At present,the treatment of meniscus injury and degeneration is not ideal,and the emergence of tissue engineering provides a new strategy for its treatment.The specific method is to implant seed cells into the meniscus

关 键 词：半月板 纤维软骨细胞 骨髓间充质干细胞 转化生长因子Β TGF-Β1 TGF-Β3 透明软骨细胞 细胞分化 组织工程 机制 综述 

分 类 号：R459.9[医药卫生—治疗学] R363[医药卫生—临床医学] R605