Structural characteristics,anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos  

作　　者：Xiaofeng Zhang Zhenhua Liang Geoffrey Ivan Neil Waterhouse Shengjun Jiang Dongxiao Sun-Waterhouse Jinmei Wang Changyang Ma Wenyi Kang 

机构地区：[1]National R&D Center for Edible Fungus Processing Technology,Henan University,Kaifeng 475004,China [2]Joint International Research Laboratory of Food&Medicine Resource Function,Henan Province,Kaifeng 475004,China [3]School of Chemical Sciences,University of Auckland,Auckland 1142,New Zealand [4]Kaifeng Key Laboratory of Functional Components in Health Food,Kaifeng 475004,China

出　　处：《Food Science and Human Wellness》2023年第2期407-415,共9页食品科学与人类健康（英文）

基　　金：supported by Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006);Key Project in Science and Technology Agency of Henan Province(202102110283 and 202102110149)。

摘　　要：This is the first report on a polysaccharide(RCJ2-Ib)isolated from Rosa Chinensis Flos.RCJ2-Ib was obtained through the extraction with water,precipitation with ethanol,separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 column.GC,FT-IR and NMR analyses revealed that RCJ2-Ib(3.3 k Da)was a 1,4-linked polymannuronic acid containing substantialβ-Danomers units.The anticoagulant effect of RCJ2-Ib evaluated by using rabbit ear venous blood and an acute blood stasis rat model showed that RCJ2-Ib had obvious anticoagulant activity in regulating endogenous and exogenous coagulation pathways and reducing serum thromboxane B2 and endothelin-1.In addition,RCJ2-Ib could also increase the number of Lactobacillus and Escherichia coli.As a result,RCJ2-Ib has the potential to inhibit thrombosis and maintain the intestinal environment.

关 键 词：Rosa Chinensis Flos Polysaccharide isolation Antithrombotic activity Intestinal flora 

分 类 号：R28[医药卫生—中药学]