A heteropolysaccharide from Rhodiola rosea L.:preparation,purification and anti-tumor activities in H22-bearing mice  

作　　者：Yaru Wu Qing Wang Huiping Liu Lulu Niu Mengyu Li Qi Jia 

机构地区：[1]State Key Laboratory of Food Nutrition and Safety,Key Laboratory of Food Nutrition and Safety,Ministry of Education of China,College of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457,China

出　　处：《Food Science and Human Wellness》2023年第2期536-545,共10页食品科学与人类健康（英文）

基　　金：the National Natural Science Foundation of China(31801568);the Natural Science Foundation of Tianjin City of China(18JCQNJC79300);the Science and Technology Major Special Projects and Engineering of Tianjin City(17ZXYENC00010);the Science and Technology Project of Gaoyou City,Jiangsu Province(GY201812)。

摘　　要：Numerous polysaccharides isolated from plants have been used to augment traditional drugs in the treatment of cancer.In order to explore the influence to hepatocellular carcinoma,a novel cold water-soluble polysaccharide was separated from Rhodiola rosea L.root(RLP)and then its structure and anti-cancer activities were tested.The chemical compositions and high performance gel permeation chromatography(HPGPC)results indicated that RLP was an acid heteropolysaccharide with the molecular weight of about1.15×10~6 Da.Furthermore,ion chromatography(IC),Fourier transform infrared(FT-IR)and nuclear magnetic resoance(NMR)further indicated that RLP was main composed of→2,4)-α-Rha(1→,→5)-α-L-Araf-(1→,α-D-Glu,→6)-β-D-Galp-(1→,β-D-Man and→4)-α-GalpA-(1→.In vivo antitumor activities of RLP were carried out by using H22 tumor-bearing mice model.The results shown that RLP(100 and 300 mg/kg)could inhibit tumor growth of H22 cells from 23.59%to 45.52%and protect thymuses and spleen without damage.In addition,according to cell cycle,AV-FITC/PI and JC-1,RLP could induce dose-dependent apopto sis of H22 cells via S phase arrested which was through a mitochondrial related pathway.Our data indicated that RLP has a broader application prospect in anti-tumor preparations.

关 键 词：POLYSACCHARIDE Antitumor activity Rhodiola rosea L. Structure analysis 

分 类 号：R285.5[医药卫生—中药学]